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Drosophila CPEB, Orb2, a Putative Biochemical Engram of Long-term Memory
dc.contributor.advisor | Si, Kausik | |
dc.contributor.advisor | Cheney, Paul | |
dc.contributor.author | Li, Liying | |
dc.date.accessioned | 2018-10-22T15:47:31Z | |
dc.date.available | 2018-10-22T15:47:31Z | |
dc.date.issued | 2017-08-31 | |
dc.date.submitted | 2017 | |
dc.identifier.other | http://dissertations.umi.com/ku:15506 | |
dc.identifier.uri | http://hdl.handle.net/1808/26893 | |
dc.description.abstract | How a transient experience creates an enduring yet dynamic memory remains a fundamental unresolved issue in studies of memory. Experience-dependent aggregation of the RNA-binding protein CPEB/Orb2 is one of the candidate mechanisms of memory maintenance. Here, using tools that allow rapid and reversible inactivation of Orb2 protein I find that Orb2 activity is required for encoding and recall of memory. Blocking the Orb2 oligomerization process by interfering with the protein phosphorylation pathway or expressing an anti-amyloidogenic peptide impairs long-term memory. Facilitating Orb2 aggregation by a DNA-J family chaperone, JJJ2, enhances the animal’s capacity to form long-term memory. Finally, I have developed tools to visualize training-dependent aggregation of Orb2. I find that aggregated Orb2 in subset of mushroom body neurons can serve as a “molecular signature” of memory and predict memory strength. My data indicate that self-sustaining aggregates of Orb2 may serve as a physical substrate of memory and provide a molecular basis for the perduring yet malleable nature of memory. | |
dc.format.extent | 154 pages | |
dc.language.iso | en | |
dc.publisher | University of Kansas | |
dc.rights | Copyright held by the author. | |
dc.subject | Neurosciences | |
dc.subject | Molecular biology | |
dc.subject | CPEB | |
dc.subject | engram | |
dc.subject | long-term memory | |
dc.subject | prion-like molecule | |
dc.title | Drosophila CPEB, Orb2, a Putative Biochemical Engram of Long-term Memory | |
dc.type | Dissertation | |
dc.contributor.cmtemember | Piotrowski, Tatjana | |
dc.contributor.cmtemember | Nishimune, Hiroshi | |
dc.contributor.cmtemember | Gibson, Matthew | |
dc.thesis.degreeDiscipline | Molecular & Integrative Physiology | |
dc.thesis.degreeLevel | Ph.D. | |
dc.identifier.orcid | ||
dc.rights.accessrights | openAccess |
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Dissertations [4889]
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KU Med Center Dissertations and Theses [464]