ATTENTION: The software behind KU ScholarWorks is being upgraded to a new version. Starting July 15th, users will not be able to log in to the system, add items, nor make any changes until the new version is in place at the end of July. Searching for articles and opening files will continue to work while the system is being updated. If you have any questions, please contact Marianne Reed at .

Show simple item record

dc.contributor.authorRoy, Anuradha
dc.contributor.authorMir, Mohammad A.
dc.identifier.citationRoy, A., & Mir, M. A. (2017). Development of High-Throughput Screening Assay for Antihantaviral Therapeutics. SLAS Discovery : Advancing Life Sciences R & D, 22(6), 767–774.
dc.description.abstractHumans acquire hantavirus infection by the inhalation of aerosolized excreta of infected rodent hosts. There is no treatment for hantavirus diseases at present. Therapeutic intervention during early stages of viral infection can improve the outcome of this zoonotic viral illness. The interaction between an evolutionary conserved sequence at the 5′ terminus of hantaviral genomic RNA and hantavirus nucleocapsid protein plays a critical role in the hantavirus replication cycle. This unique interaction is a novel target for therapeutic intervention of hantavirus disease. We developed a very sensitive, tractable, and cost-effective fluorescence-based assay to monitor the interaction between the nucleocapsid protein and the target RNA sequence. The assay was optimized for high-throughput screening of chemical libraries to identify molecules that interrupt this RNA–protein interaction. The assay was validated using a library of 6880 chemical compounds. This validation screen demonstrated the reproducibility and validity of required statistical criteria for high-throughput screening. The assay is ready to use for high-throughput screening of large chemical libraries to identify antihantaviral therapeutic molecules and can be amenable to similar targets in other viruses.en_US
dc.publisherSAGE Publicationsen_US
dc.rights© 2017 Society for Laboratory Automation and Screeningen_US
dc.subjectHantavirus replicationen_US
dc.subjectNucleocapsid proteinen_US
dc.subjectHigh-throughput screenen_US
dc.subjectBunyaviridae familyen_US
dc.subjectNegative-strand RNA virusesen_US
dc.titleDevelopment of High-Throughput Screening Assay for Antihantaviral Therapeuticsen_US
kusw.kuauthorRoy, Anuradha
kusw.kudepartmentHigh Throughput Screeningen_US
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US

Files in this item


This item appears in the following Collection(s)

Show simple item record