dc.contributor.author | Franklin, Jade M. | |
dc.contributor.author | Carrasco, Gonzalo A. | |
dc.date.accessioned | 2017-06-06T20:24:47Z | |
dc.date.available | 2017-06-06T20:24:47Z | |
dc.date.issued | 2012-10 | |
dc.identifier.citation | Franklin, J. M., & Carrasco, G. A. (2012). Cannabinoid-Induced Enhanced Interaction and Protein Levels of Serotonin 5-HT2A and Dopamine D2 Receptors in Rat Prefrontal Cortex. Journal of Psychopharmacology (Oxford, England), 26(10), 1333–1347. http://doi.org/10.1177/0269881112450786 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/24395 | |
dc.description.abstract | Recent evidence suggests that non-selective cannabinoid receptor agonists may regulate serotonin 2A (5-HT2A) receptor neurotransmission in brain. The molecular mechanisms of this regulation are unknown but could involve cannabinoid-induced enhanced interaction between 5-HT2A and dopamine D2 (D2) receptors. Here, we present experimental evidence that Sprague-Dawley rats treated with a non-selective cannabinoid receptor agonist (CP55,940, 50μg/kg, 7days, i.p.) showed enhanced co-immunoprecipitation of 5-HT2A and D2 receptors and enhanced membrane-associated expression of D2 and 5-HT2A receptors in prefrontal cortex (PFCx). Furthermore, 5-HT2A receptor mRNA levels were increased in PFCx suggesting a cannabinoid-induced upregulation of 5-HT2A receptors. To date, two cannabinoids receptors have been found in brain, CB1 and CB2 receptors. We used selective cannabinoid agonists in a neuronal cell line to study mechanisms that could mediate this 5-HT2A receptor upregulation. We found that selective CB2 receptor agonists upregulate 5-HT2A receptors by a mechanism that seems to involve activation of Gαi G-proteins, ERK1/2, and AP-1 transcription factor. We hypothesize that the enhanced cannabinoid-induced interaction between 5-HT2A and D2 receptors and in 5-HT2A and D2 receptors protein levels in the PFCx might provide a molecular mechanism by which activation of cannabinoid receptors might be contribute to the pathophysiology of some cognitive and mood disorders. | en_US |
dc.publisher | SAGE Publications | en_US |
dc.subject | Serotonin | en_US |
dc.subject | Prefrontal cortex | en_US |
dc.subject | 5-HT2A receptor | en_US |
dc.subject | D2 receptor | en_US |
dc.subject | G proteins | en_US |
dc.subject | Cannabis sativa | en_US |
dc.title | Cannabinoid-Induced Enhanced Interaction and Protein Levels of Serotonin 5-HT2A and Dopamine D2 Receptors in Rat Prefrontal Cortex | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Carrasco, Gonzalo A. | |
kusw.kuauthor | Franklin, Jade M. | |
kusw.kudepartment | Pharmacy | en_US |
dc.identifier.doi | 10.1177/0269881112450786 | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC3746962 | en_US |
dc.rights.accessrights | openAccess | |