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    Clearance and Phosphorylation of Alpha-Synuclein Are Inhibited in Methionine Sulfoxide Reductase A Null Yeast Cells

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    Oien_2009.pdf (2.851Mb)
    Issue Date
    2009-11
    Author
    Oien, Derek B.
    Shinogle, Heather E.
    Moore, David S.
    Moskovitz, Jackob
    Publisher
    Humana Press
    Type
    Article
    Article Version
    Scholarly/refereed, author accepted manuscript
    Rights
    © Humana Press 2009
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    Abstract
    Aggregated α-synuclein and the point mutations Ala30Pro and Ala53Thr of α-synuclein are associated with Parkinson’s disease. The physiological roles of α-synuclein and methionine oxidation of the α-synuclein protein structure and function are not fully understood. Methionine sulfoxide reductase A (MsrA) reduces methionine sulfoxide residues and functions as an antioxidant. To monitor the effect of methionine oxidation to α-synuclein on basic cellular processes, α-synucleins were expressed in msrA null mutant and wild-type yeast cells. Protein degradation was inhibited in the α-synuclein-expressing msrA null mutant cells compared to α-synuclein-expressing wild-type cells. Increased inhibition of degradation and elevated accumulations of fibrillated proteins were observed in SynA30P-expressing msrA null mutant cells. Additionally, methionine oxidation inhibited α-synuclein phosphorylation in yeast cells and in vitro by casein kinase 2. Thus, a compromised MsrA function combined with α-synuclein overexpression may promote processes leading to synucleinopathies.
    URI
    http://hdl.handle.net/1808/24294
    DOI
    https://doi.org/10.1007/s12031-009-9274-8
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    • Pharmacy Scholarly Works [280]
    Citation
    Oien, D. B., Shinogle, H. E., Moore, D. S., & Moskovitz, J. (2009). Clearance and Phosphorylation of Alpha-Synuclein Are Inhibited in Methionine Sulfoxide Reductase A Null Yeast Cells. Journal of Molecular Neuroscience : MN, 39(3), 323–332. http://doi.org/10.1007/s12031-009-9274-8

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    KU Libraries
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    KU Libraries
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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