dc.contributor.advisor | Stobaugh, John F | |
dc.contributor.author | Johnson, Ryan Tyler | |
dc.date.accessioned | 2017-05-15T22:33:59Z | |
dc.date.available | 2017-05-15T22:33:59Z | |
dc.date.issued | 2016-12-31 | |
dc.date.submitted | 2016 | |
dc.identifier.other | http://dissertations.umi.com/ku:15017 | |
dc.identifier.uri | http://hdl.handle.net/1808/24202 | |
dc.description.abstract | Capillary electrophoresis-mass spectrometry (CE-MS) is a useful analytical technique capable of high efficiency separations from complex and low volume samples. However, the interface between CE and MS is not nearly as robust as that for LC-MS. Herein, we describe applications of CE-MS that demonstrate the technique’s utility and effectiveness. Additionally, advancements to the CE-MS interface design are presented. Firstly, we developed a CE-UV and CE-ESI-MS method that utilizes a borate background electrolyte for the investigation of 14 natural flavonoid glycones and aglycones. Borate complexes with catechol containing flavonoids were detected in MS spectra. These complexes can aid in unknown flavonoid identification as well as mass isomer differentiation of flavonoids with varying hydroxylation patterns. Secondly, we fabricated a novel sheathless CE-ESI-MS interface with better mechanical stability than other similar interfaces. The interface was capable of nM LODs and robust electrophoresis and electrospray. Lastly, the novel interface was used to develop a CE-MS method for several biomarkers of lipid peroxidation from low volume microdialysis samples. The method utilizes charge reversal derivatization to achieve superior detection limits compared to native lipid biomarkers. Post-derivatization and sample clean up, approximately 25 nM LODs were achievable. | |
dc.format.extent | 116 pages | |
dc.language.iso | en | |
dc.publisher | University of Kansas | |
dc.rights | Copyright held by the author. | |
dc.subject | Analytical chemistry | |
dc.subject | Biochemistry | |
dc.subject | Capillary Electrophoresis | |
dc.subject | Eicosanoids | |
dc.subject | Flavonoids | |
dc.subject | Interface | |
dc.subject | Mass Spectrometry | |
dc.subject | Sheathless | |
dc.title | Advances and Applications of Capillary Electrophoresis-Mass Spectrometry | |
dc.type | Dissertation | |
dc.contributor.cmtemember | Lunte, Susan | |
dc.contributor.cmtemember | Dunn, Robert | |
dc.contributor.cmtemember | Desaire, Heather | |
dc.contributor.cmtemember | Siahaan, Teruna | |
dc.thesis.degreeDiscipline | Chemistry | |
dc.thesis.degreeLevel | Ph.D. | |
dc.identifier.orcid | | |
dc.rights.accessrights | openAccess | |