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dc.contributor.advisorStobaugh, John F
dc.contributor.authorJohnson, Ryan Tyler
dc.date.accessioned2017-05-15T22:33:59Z
dc.date.available2017-05-15T22:33:59Z
dc.date.issued2016-12-31
dc.date.submitted2016
dc.identifier.otherhttp://dissertations.umi.com/ku:15017
dc.identifier.urihttp://hdl.handle.net/1808/24202
dc.description.abstractCapillary electrophoresis-mass spectrometry (CE-MS) is a useful analytical technique capable of high efficiency separations from complex and low volume samples. However, the interface between CE and MS is not nearly as robust as that for LC-MS. Herein, we describe applications of CE-MS that demonstrate the technique’s utility and effectiveness. Additionally, advancements to the CE-MS interface design are presented. Firstly, we developed a CE-UV and CE-ESI-MS method that utilizes a borate background electrolyte for the investigation of 14 natural flavonoid glycones and aglycones. Borate complexes with catechol containing flavonoids were detected in MS spectra. These complexes can aid in unknown flavonoid identification as well as mass isomer differentiation of flavonoids with varying hydroxylation patterns. Secondly, we fabricated a novel sheathless CE-ESI-MS interface with better mechanical stability than other similar interfaces. The interface was capable of nM LODs and robust electrophoresis and electrospray. Lastly, the novel interface was used to develop a CE-MS method for several biomarkers of lipid peroxidation from low volume microdialysis samples. The method utilizes charge reversal derivatization to achieve superior detection limits compared to native lipid biomarkers. Post-derivatization and sample clean up, approximately 25 nM LODs were achievable.
dc.format.extent116 pages
dc.language.isoen
dc.publisherUniversity of Kansas
dc.rightsCopyright held by the author.
dc.subjectAnalytical chemistry
dc.subjectBiochemistry
dc.subjectCapillary Electrophoresis
dc.subjectEicosanoids
dc.subjectFlavonoids
dc.subjectInterface
dc.subjectMass Spectrometry
dc.subjectSheathless
dc.titleAdvances and Applications of Capillary Electrophoresis-Mass Spectrometry
dc.typeDissertation
dc.contributor.cmtememberLunte, Susan
dc.contributor.cmtememberDunn, Robert
dc.contributor.cmtememberDesaire, Heather
dc.contributor.cmtememberSiahaan, Teruna
dc.thesis.degreeDisciplineChemistry
dc.thesis.degreeLevelPh.D.
dc.identifier.orcid
dc.rights.accessrightsopenAccess


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