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Molecular landscape of the ribosome pre-initiation complex during mRNA scanning: structural role for eIF3c and its control by eIF5
dc.contributor.author | Obayashi, Eiji | |
dc.contributor.author | Luna, Rafael E. | |
dc.contributor.author | Nagata, Takashi | |
dc.contributor.author | Martin-Marcos, Pilar | |
dc.contributor.author | Hiraishi, Hiroyuki | |
dc.contributor.author | Singh, Chingakham Ranjit | |
dc.contributor.author | Erzberger, Jan Peter | |
dc.contributor.author | Zhang, Fan | |
dc.contributor.author | Arthanari, Haribabu | |
dc.contributor.author | Morris, Jacob | |
dc.contributor.author | Pellarin, Riccardo | |
dc.contributor.author | Moore, Chelsea | |
dc.contributor.author | Harmon, Ian | |
dc.contributor.author | Ananbandam, Asokan | |
dc.contributor.author | Gao, Philip | |
dc.date.accessioned | 2017-05-12T16:59:55Z | |
dc.date.available | 2017-05-12T16:59:55Z | |
dc.date.issued | 2017-04-06 | |
dc.identifier.citation | Obayashi, E., Luna, R. E., Nagata, T., Martin-Marcos, P., Hiraishi, H., Singh, C. R., … Asano, K. (2017). Molecular landscape of the ribosome pre-initiation complex during mRNA scanning: structural role for eIF3c and its control by eIF5. Cell Reports, 18(11), 2651–2663. http://doi.org/10.1016/j.celrep.2017.02.052 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/24116 | |
dc.description.abstract | During eukaryotic translation initiation, eIF3 binds the solvent-accessible side of the 40S ribosome and recruits the gate-keeper protein eIF1 and eIF5 to the decoding center. This is largely mediated by the N-terminal domain (NTD) of eIF3c, which can be divided into three parts: 3c0, 3c1 and 3c2. The N-terminal part, 3c0, binds eIF5 strongly, but only weakly to the ribosome-binding surface of eIF1, whereas 3c1 and 3c2 form a stoichiometric complex with eIF1. 3c1 contacts eIF1 through Arg-53 and Leu-96, while 3c2 faces 40S protein uS15/S13, to anchor eIF1 to the scanning pre-initiation complex (PIC). We propose that the 3c0:eIF1 interaction diminishes eIF1 binding to the 40S, whereas 3c0:eIF5 interaction stabilizes the scanning PIC by precluding this inhibitory interaction. Upon start codon recognition, interactions involving eIF5, and ultimately 3c0:eIF1 association facilitate eIF1 release. Our results reveal intricate molecular interactions within the PIC, programmed for rapid scanning-arrest at the start codon. | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 4.0 (CC BY-NC-ND 4.0), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en_US |
dc.title | Molecular landscape of the ribosome pre-initiation complex during mRNA scanning: structural role for eIF3c and its control by eIF5 | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Ananbandam, Asokan | |
kusw.kuauthor | Gao, Philip | |
kusw.kudepartment | Higuchi Biosciences Center | en_US |
dc.identifier.doi | 10.1016/j.celrep.2017.02.052 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC5382721 | en_US |
dc.rights.accessrights | openAccess |
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Except where otherwise noted, this item's license is described as: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 4.0 (CC BY-NC-ND 4.0), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.