ATTENTION: The software behind KU ScholarWorks is being upgraded to a new version. Starting July 15th, users will not be able to log in to the system, add items, nor make any changes until the new version is in place at the end of July. Searching for articles and opening files will continue to work while the system is being updated.
If you have any questions, please contact Marianne Reed at mreed@ku.edu .
The Effects of PVP(Fe(III)) Catalyst on Polymer Molecular Weight and Gene Delivery via Biodegradable Cross-Linked Polyethylenimine
dc.contributor.author | Shum, Victor W. T. | |
dc.contributor.author | Gabrielson, Nathan P. | |
dc.contributor.author | Forrest, M. Laird | |
dc.contributor.author | Pack, Daniel W. | |
dc.date.accessioned | 2017-05-10T19:50:53Z | |
dc.date.available | 2017-05-10T19:50:53Z | |
dc.date.issued | 2011-09-03 | |
dc.identifier.citation | Shum, Victor W.T. et al. “The Effects of PVP(Fe(III)) Catalyst on Polymer Molecular Weight and Gene Delivery via Biodegradable Cross-Linked Polyethylenimine.” Pharmaceutical research 29.2 (2012): 500–510. | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/24087 | |
dc.description | The original publication is available at www.springerlink.com | en_US |
dc.description.abstract | PurposeCrosslinked, degradable derivatives of low-molecular-weight polyethylenimine (PEI) are relatively efficient and non-cytotoxic gene delivery agents. To further investigate these promising materials, a new synthetic approach was developed using a poly(4-vinylpyridine)-supported Fe(III) catalyst (PVP(Fe(III))) that provides more facile synthesis and enhanced control of polymer molecular weight.MethodsBiodegradable polymers (D.PEI) comprising 800-Da PEI crosslinked with 1,6-hexanediol diacrylate and exhibiting molecular weights of 1.2, 6.2, and 48 kDa were synthesized utilizing the PVP(Fe(III)) catalyst. D.PEI/DNA polyplexes were characterized using gel retardation, ethidium bromide exclusion, heparan sulfate displacement, and dynamic light scattering. In vitro transfection, cellular uptake, and cytotoxicity of the polyplexes were tested in human cervical cancer cells (HeLa) and human breast cancer cells (MDA-MB-231).ResultsD.PEIs tightly complexed plasmid DNA and formed 320- to 440-nm diameter polyplexes, similar to those comprising non-degradable, 25-kDa, branched PEI. D.PEI polyplexes mediated 2- to 5-fold increased gene delivery efficacy compared to 25-kDa PEI and exhibited 20% lower cytotoxicity in HeLa and no toxicity in MDA-MB-231. In addition, 2- to 7-fold improved cellular uptake of DNA was achieved with D.PEI polyplexes.ConclusionsPVP(Fe(III)) catalyst provided a more controlled synthesis of D.PEIs, and these materials demonstrated improved in vitro transfection efficacy and reduced cytotoxicity. | en_US |
dc.publisher | American Association of Pharmaceutical Scientists | en_US |
dc.subject | Polyethylenimine | en_US |
dc.subject | Non-viral gene delivery | en_US |
dc.subject | Biodegradable polymer | en_US |
dc.subject | Polymer supported ferric chloride | en_US |
dc.title | The Effects of PVP(Fe(III)) Catalyst on Polymer Molecular Weight and Gene Delivery via Biodegradable Cross-Linked Polyethylenimine | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Forrest, M. Laird | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
dc.identifier.doi | 10.1007/s11095-011-0576-5 | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC3465843 | en_US |
dc.rights.accessrights | openAccess |