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    Reconfiguration of the proteasome during chaperone-mediated assembly

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    Lovell_2013.pdf (2.720Mb)
    Issue Date
    2013-05-23
    Author
    Park, Soyeon
    Li, Xueming
    Kim, Ho Min
    Singh, Chingakham Ranjit
    Tian, Geng
    Hoyt, Martin A.
    Lovell, Scott
    Battaile, Michal
    Coffino, Philip
    Roelofs, Jeroen
    Cheng, Yifan
    Finley, Daniel
    Publisher
    Nature Publishing Group
    Type
    Article
    Article Version
    Scholarly/refereed, author accepted manuscript
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    Abstract
    The proteasomal ATPase ring, comprising Rpt1-Rpt6, associates with the heptameric α ring of the proteasome core particle (CP) in the mature proteasome, with the Rpt C-terminal tails inserting into pockets of the α ring1–4. Rpt ring assembly is mediated by four chaperones, each binding a distinct Rpt subunit5–10. We report that the base subassembly of the proteasome, which includes the Rpt ring, forms a high affinity complex with the CP. This complex is subject to active dissociation by the chaperones Hsm3, Nas6, and Rpn14. Chaperone-mediated dissociation was abrogated by a nonhydrolyzable ATP analog, indicating that chaperone action is coupled to nucleotide hydrolysis by the Rpt ring. Unexpectedly, synthetic Rpt tail peptides bound α pockets with poor specificity, except for Rpt6, which uniquely bound the α2/α3 pocket. Although the Rpt6 tail is not visualized within an α pocket in mature proteasomes2–4, it inserts into the α2/α3 pocket in the base-CP complex and is important for complex formation. Thus, the Rpt-CP interface is reconfigured when the lid complex joins the nascent proteasome to form the mature holoenzyme.
    URI
    http://hdl.handle.net/1808/23830
    DOI
    https://doi.org/10.1038/nature12123
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    • Higuchi Biosciences Center Scholarly Works [54]
    Citation
    Park, S., Li, X., Kim, H. M., Singh, C. R., Tian, G., Hoyt, M. A., … Finley, D. (2013). Reconfiguration of the proteasome during chaperone-mediated assembly. Nature, 497(7450), 512–516. http://doi.org/10.1038/nature12123

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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