Structural and Inhibitor Studies of Norovirus 3C-like Proteases
dc.contributor.author | Takahashi, Daisuke | |
dc.contributor.author | Kim, Yunjeong | |
dc.contributor.author | Lovell, Scott | |
dc.contributor.author | Prakash, Om | |
dc.contributor.author | Chang, Kyeong-Ok | |
dc.date.accessioned | 2017-04-10T20:29:04Z | |
dc.date.available | 2017-04-10T20:29:04Z | |
dc.date.issued | 2013-09-17 | |
dc.identifier.citation | Takahashi, Daisuke et al. “Structural and Inhibitor Studies of Norovirus 3C-like Proteases.” Virus research 178.2 (2013): 10.1016/j.virusres.2013.09.008. | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/23618 | |
dc.description.abstract | Noroviruses have a single-stranded, positive sense 7–8 kb RNA genome, which encodes a polyprotein precursor processed by a virus-encoded 3C-like cysteine protease (3CLpro) to generate mature non-structural proteins. Because processing of the polyprotein is essential for virus replication, norovirus 3CLpro has been targeted for the discovery of anti-norovirus small molecule therapeutics. Thus, we performed functional, structural and inhibition studies of norovirus 3CLpro with fluorescence resonance energy transfer (FRET) assay, X-ray crystallography, and NMR spectroscopy with a synthetic protease inhibitor. Three 3CLpro from Norwalk virus (NV, genogroup I), MD145 (genogroup II) and murine norovirus-1 (MNV-1, genogroup V) were optimized for a FRET assay, and compared for the inhibitory activities of a synthetic protease inhibitor (GC376). The apo 3D structures of NV 3CLpro determined with X-ray crystallography and NMR spectroscopy were further analyzed. In addition, the binding mode of NV 3CLpro-GC376 was compared with X-ray crystallography and NMR spectroscopy. The results of this report provide insight into the interaction of NV 3CLpro with substrate/inhibitor for better understanding of the enzyme and antiviral drug development. | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 3.0 (CC BY-NC-ND 3.0 US), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | |
dc.title | Structural and Inhibitor Studies of Norovirus 3C-like Proteases | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Lovell, Scott | |
kusw.kudepartment | Higuchi Biosciences Center | en_US |
dc.identifier.doi | 10.1016/j.virusres.2013.09.008 | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess |
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