ATTENTION: The software behind KU ScholarWorks is being upgraded to a new version. Starting July 15th, users will not be able to log in to the system, add items, nor make any changes until the new version is in place at the end of July. Searching for articles and opening files will continue to work while the system is being updated.
If you have any questions, please contact Marianne Reed at mreed@ku.edu .
The Macroscopic Rate of Nucleic Acid Translocation by Hepatitis C virus Helicase NS3h is Dependent on Both the Sugar and Base Moieties
dc.contributor.author | Khaki, Ali R. | |
dc.contributor.author | Field, Cassandra | |
dc.contributor.author | Malik, Shuja Shafi | |
dc.contributor.author | Niedziela-Majka, Anita | |
dc.contributor.author | Leavitt, Stephanie A. | |
dc.contributor.author | Wang, Ruth | |
dc.contributor.author | Hung, Magdeleine | |
dc.contributor.author | Sakowicz, Roman | |
dc.contributor.author | Brendza, Katherine M. | |
dc.contributor.author | Fischer, Christopher J. | |
dc.date.accessioned | 2017-03-27T20:57:46Z | |
dc.date.available | 2017-03-27T20:57:46Z | |
dc.date.issued | 2010-05-06 | |
dc.identifier.citation | Khaki, Ali R., Cassandra Field, Shuja Malik, Anita Niedziela-Majka, Stephanie A. Leavitt, Ruth Wang, Magdeleine Hung, Roman Sakowicz, Katherine M. Brendza, and Christopher J. Fischer. "The Macroscopic Rate of Nucleic Acid Translocation by Hepatitis C Virus Helicase NS3h Is Dependent on Both Sugar and Base Moieties." Journal of Molecular Biology 400.3 (2010): 354-78. | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/23490 | |
dc.description.abstract | The NS3 helicase (NS3h) of hepatitis C virus (HCV) is a 3′ to 5′ SF2 RNA and DNA helicase that is essential for the replication of HCV. We have examined the kinetic mechanism of translocation of NS3h along single-stranded nucleic acid with bases rU, dU and dT and have found that the macroscopic rate of translocation is dependent upon both the base and sugar moieties of the nucleic acid, with approximate macroscopic translocation rates of 3 nt/s (oligo-dT), 35 nt/s (oligo-dU), and 42 nt/s (oligo-rU), respectively. We found a strong correlation between the macroscopic translocation rates and the binding affinity of the translocating NS3h protein to the respective substrates such that weaker affinity corresponded to faster translocation. The values of K0.5 for NS3h translocation at a saturating ATP concentration are: (3.3 ± 0.4) μM nucleotide (poly-dT), (27 ± 2) μM nucleotide (poly-dU), and (36 ± 2) μM nucleotide (poly-rU). Furthermore, the results of isothermal titration of NS3h with these oligonucleotides suggest that differences in TΔS° are the principal source of the differences in the affinity of NS3h binding to these substrates. Interestingly, despite the differences in macroscopic translocation rates and binding affinities, the ATP coupling stoichiometry for NS3h translocation was identical for all three substrates, ~0.5 ATP molecules consumed per nucleotide translocated. This similar periodicity of ATP consumption implies a similar mechanism for NS3h translocation along RNA and DNA substrates. | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 3.0 (CC BY-NC-ND 3.0 US), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | |
dc.subject | NS3 helicase | en_US |
dc.subject | Translocation | en_US |
dc.subject | Nucleic acid | en_US |
dc.subject | ATPase | en_US |
dc.subject | Mechanism | en_US |
dc.title | The Macroscopic Rate of Nucleic Acid Translocation by Hepatitis C virus Helicase NS3h is Dependent on Both the Sugar and Base Moieties | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Fischer, Christopher J. | |
kusw.kudepartment | Physics and Astronomy | en_US |
dc.identifier.doi | 10.1016/j.jmb.2010.04.065 | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's license is described as: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 3.0 (CC BY-NC-ND 3.0 US), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.