dc.contributor.author | Gupta, Vineet | |
dc.contributor.author | Lyne, Dina V. | |
dc.contributor.author | Barragan, Marilyn | |
dc.contributor.author | Berkland, Cory J. | |
dc.contributor.author | Detamore, Michael S. | |
dc.date.accessioned | 2017-03-08T20:18:10Z | |
dc.date.available | 2017-03-08T20:18:10Z | |
dc.date.issued | 2016-07-27 | |
dc.identifier.citation | Gupta, V., Lyne, D. V., Barragan, M., Berkland, C. J., & Detamore, M. S. (2016). Microsphere-Based Scaffolds Encapsulating Tricalcium Phosphate And Hydroxyapatite For Bone Regeneration. Journal of Materials Science. Materials in Medicine, 27(7), 121. http://doi.org/10.1007/s10856-016-5734-1 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/23370 | |
dc.description.abstract | Bioceramic mixtures of tricalcium phosphate (TCP) and hydroxyapatite (HAp) are widely used for bone regeneration because of their excellent cytocompatibility, osteoconduction, and osteoinduction. Therefore, we hypothesized that incorporation of a mixture of TCP and HAp in microsphere-based scaffolds would enhance osteogenesis of rat bone marrow stromal cells (rBMSCs) compared to a positive control of scaffolds with encapsulated bone-morphogenic protein-2 (BMP-2). Poly(D,L-lactic-co-glycolic acid) (PLGA) microsphere-based scaffolds encapsulating TCP and HAp mixtures in two different ratios (7:3 and 1:1) were fabricated with the same net ceramic content (30 wt%) to evaluate how incorporation of these ceramic mixtures would affect the osteogenesis in rBMSCs. Encapsulation of TCP/HAp mixtures impacted microsphere morphologies and the compressive moduli of the scaffolds. Additionally, TCP/HAp mixtures enhanced the end-point secretion of extracellular matrix (ECM) components relevant to bone tissue compared to the “blank” (PLGA-only) microsphere-based scaffolds as evidenced by the biochemical, gene expression, histology, and immunohistochemical characterization. Moreover, the TCP/HAp mixture groups even surpassed the BMP-2 positive control group in some instances in terms of matrix synthesis and gene expression. Lastly, gene expression data suggested that the rBMSCs responded differently to different TCP/HAp ratios presented to them. Altogether, it can be concluded that TCP/HAp mixtures stimulated the differentiation of rBMSCs toward an osteoblastic phenotype, and therefore may be beneficial in gradient microsphere-based scaffolds for osteochondral regeneration. | en_US |
dc.publisher | Springer Verlag | en_US |
dc.subject | Tricalcium phosphate | en_US |
dc.subject | Hydroxyapatite | en_US |
dc.subject | Biphasic calcium phosphates | en_US |
dc.subject | Microsphere-based scaffold | en_US |
dc.subject | Bone regeneration | en_US |
dc.title | Microsphere-Based Scaffolds Encapsulating Tricalcium Phosphate And Hydroxyapatite For Bone Regeneration | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Gupta, Vineet | |
kusw.kuauthor | Lyne, Dina V. | |
kusw.kuauthor | Barragan, Marilyn | |
kusw.kuauthor | Berkland, Cory J. | |
kusw.kuauthor | Detamore, Michael S. | |
kusw.kudepartment | Chemistry | en_US |
dc.identifier.doi | 10.1007/s10856-016-5734-1 | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess | |