Estradiol-induced desensitization of 5-HT1A receptor signaling in the paraventricular nucleus of the hypothalamus is independent of estrogen receptor-beta
dc.contributor.author | Rossi, Dania | |
dc.contributor.author | Dai, Ying | |
dc.contributor.author | Thomas, Peter | |
dc.contributor.author | Carrasco, Gonzalo A. | |
dc.contributor.author | DonCarlos, Lydia L. | |
dc.contributor.author | Muma, Nancy A. | |
dc.contributor.author | Li, Qian | |
dc.date.accessioned | 2017-02-22T20:04:07Z | |
dc.date.available | 2017-02-22T20:04:07Z | |
dc.date.issued | 2010-08 | |
dc.identifier.citation | Rossi, D. V., Dai, Y., Thomas, P., Carrasco, G. A., DonCarlos, L. L., Muma, N. A., & Li, Q. (2010). Estradiol-induced desensitization of 5-HT1A receptor signaling in the paraventricular nucleus of the hypothalamus is independent of estrogen receptor-beta. Psychoneuroendocrinology, 35(7), 1023–1033. http://doi.org/10.1016/j.psyneuen.2010.01.003 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/23220 | |
dc.description.abstract | Estradiol regulates serotonin 1A(5-HT1A) receptor signaling. Since desensitization of 5-HT1A receptors may be an underlying mechanism by which selective serotonin reuptake inhibitors (SSRIs) mediate their therapeutic effects and combining estradiol with SSRIs enhances the efficacy of the SSRIs, it is important to determine which estrogen receptors are capable of desensitizating 5-HT1A receptor function. We previously demonstrated that selective activation of the estrogen receptor, GPR30, desensitizes 5-HT1A receptor signaling in rat hypothalamic paraventricular nucleus(PVN). However, since estrogen receptor beta(ERβ), is highly expressed in the PVN, we investigated the role of ERβ in estradiol-induced desensitization of 5-HT1A receptor signaling. We first showed that a selective ERβ agonist, diarylpropionitrile(DPN) has a 100-fold lower binding affinity than estradiol for GPR30. Administration of DPN did not desensitize 5-HT1A receptor signaling in rat PVN as demonstrated by agonist-stimulated hormone release. Second, we used a recombinant adenovirus containing ERβ siRNAs to decrease ERβ expression in the PVN. Reductions in ERβ did not alter the estradiol-induced desensitization of 5-HT1A receptor signaling in oxytocin cells. In contrast, in animals with reduced ERβ, estradiol administration, instead of producing desensitization, augmented the ACTH response to a 5-HT1A agonist. Combined with the results from the DPN treatment experiments, desensitization of 5-HT1A receptor signaling does not appear to be mediated by ERβ in oxytocin cells, but that ERβ, together with GPR30, may play a complex role in central regulation of 5-HT1A-mediated ACTH release. Determining the mechanisms by which estrogens induce desensitization may aid in the development of better treatments for mood disorders. | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 3.0 (CC BY-NC-ND 3.0 US), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en_US |
dc.subject | Estrogen receptors | en_US |
dc.subject | Neuroendocrine responses | en_US |
dc.subject | siRNA | en_US |
dc.subject | Recombinant adenovirus | en_US |
dc.subject | Serotonin receptors | en_US |
dc.subject | Mood disorders | en_US |
dc.subject | Estradiol | en_US |
dc.title | Estradiol-induced desensitization of 5-HT1A receptor signaling in the paraventricular nucleus of the hypothalamus is independent of estrogen receptor-beta | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Rossi, Dania V. | |
kusw.kuauthor | Dai, Ying | |
kusw.kuauthor | Thomas, Peter | |
kusw.kuauthor | Carrasco, Gonzalo A. | |
kusw.kuauthor | DonCarlos, Lydia L. | |
kusw.kuauthor | Muma, Nancy A. | |
kusw.kuauthor | Li, Qian | |
kusw.kudepartment | Phamacology and Toxicology | en_US |
dc.identifier.doi | 10.1016/j.psyneuen.2010.01.003 | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess |
Files in this item
This item appears in the following Collection(s)
-
Pharmacy Scholarly Works [293]
Except where otherwise noted, this item's license is described as: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 3.0 (CC BY-NC-ND 3.0 US), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.