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dc.contributor.authorJiang, Lei
dc.contributor.authorBechtel, Misty D.
dc.contributor.authorBean, Jennifer L.
dc.contributor.authorWinefield, Robert D.
dc.contributor.authorWilliams, Todd D.
dc.contributor.authorZaidi, Asma
dc.contributor.authorMichaelis, Elias K.
dc.contributor.authorMichaelis, Mary Lou
dc.date.accessioned2017-02-01T20:36:32Z
dc.date.available2017-02-01T20:36:32Z
dc.date.issued2014-01-14
dc.identifier.citationJiang, Lei, Misty D. Bechtel, Jennifer L. Bean, Robert Winefield, Todd D. Williams, Asma Zaidi, Elias K. Michaelis, and Mary L. Michaelis. "Effects of Gangliosides on the Activity of the Plasma Membrane Ca2 -ATPase." Biochimica Et Biophysica Acta (BBA) - Biomembranes 1838.5 (2014): 1255-265.en_US
dc.identifier.urihttp://hdl.handle.net/1808/22718
dc.description.abstractControl of intracellular calcium concentrations ([Ca2+]i) is essential for neuronal function, and the plasma membrane Ca2+-ATPase (PMCA) is crucial for the maintenance of low [Ca2+]i. We previously reported on loss of PMCA activity in brain synaptic membranes during aging. Gangliosides are known to modulate Ca2+ homeostasis and signal transduction in neurons. In the present study, we observed age-related changes in the ganglioside composition of synaptic plasma membranes. This led us to hypothesize that alterations in ganglioside species might contribute to the age-associated loss of PMCA activity. To probe the relationship between changes in endogenous ganglioside content or composition and PMCA activity in membranes of cortical neurons, we induced depletion of gangliosides by treating neurons with D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (D-PDMP). This caused a marked decrease in the activity of PMCA, which suggested a direct correlation between ganglioside content and PMCA activity. Neurons treated with neuraminidase exhibited an increase in GM1 content, a loss in poly-sialoganglioside content, and a decrease in PMCA activity that was greater than that produced by D-PDMP treatment. Thus, it appeared that poly-sialogangliosides had a stimulatory effect whereas mono-sialogangliosides had the opposite effect. Our observations add support to previous reports of PMCA regulation by gangliosides by demonstrating that manipulations of endogenous ganglioside content and species affect the activity of PMCA in neuronal membranes. Furthermore, our studies suggest that age-associated loss in PMCA activity may result in part from changes in the lipid environment of this Ca2+ transporter.en_US
dc.publisherElsevieren_US
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 3.0 (CC BY-NC-ND 3.0 US), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subjectAgingen_US
dc.subjectGangliosidesen_US
dc.subjectPlasma membrane Ca2+-ATPaseen_US
dc.subjectCalciumen_US
dc.subjectNeuraminidaseen_US
dc.subjectD(L)-threo-1-phenl-2-decanoylamino-3-morpholino-1-propanol (D-PDMP)en_US
dc.titleEffects of Gangliosides on the Activity of the Plasma Membrane Ca2+-ATPaseen_US
dc.typeArticleen_US
kusw.kuauthorWinefield, Robert
kusw.kudepartmentPharmacology & Toxicologyen_US
dc.identifier.doi10.1016/j.bbamem.2014.01.003en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-0944-5407
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccess


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This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 3.0 (CC BY-NC-ND 3.0 US), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Except where otherwise noted, this item's license is described as: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 3.0 (CC BY-NC-ND 3.0 US), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.