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Kinetic Mechanism of DNA Translocation by the RSC Molecular Motor

dc.contributor.authorEastlund, Allen
dc.contributor.authorMalik, Shuja Shafi
dc.contributor.authorFischer, Christopher J.
dc.date.accessioned2017-01-18T19:19:23Z
dc.date.available2017-01-18T19:19:23Z
dc.date.issued2013-02-09
dc.identifier.citationEastlund, Allen, Shuja Shafi Malik, and Christopher J. Fischer. "Kinetic Mechanism of DNA Translocation by the RSC Molecular Motor." Archives of Biochemistry and Biophysics 532.2 (2013): 73-83.en_US
dc.identifier.urihttp://hdl.handle.net/1808/22643
dc.description.abstractATP-dependent nucleosome repositioning by chromatin remodeling enzymes requires the translocation of these enzymes along the nucleosomal DNA. Using a fluorescence stopped-flow assay we monitored DNA translocation by a minimal RSC motor and through global analysis of these time courses we have determined that this motor has a macroscopic translocation rate of 2.9 bp/s with a step size of 1.24 bp. From the complementary quantitative analysis of the associated time courses of ATP consumption during DNA translocation we have determined that this motor has an efficiency of 3.0 ATP/bp, which is slightly less that the efficiency observed for several genetically related DNA helicases and which likely results from random pausing by the motor during translocation. Nevertheless, this motor is able to exert enough force during translocation to displace streptavidin from biotinylated DNA. Taken together these results are the necessary first step for quantifying both the role of DNA translocation in nucleosome repositioning by RSC and the efficiency at which RSC couples ATP binding and hydrolysis to nucleosome repositioning.en_US
dc.publisherElsevieren_US
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 3.0 (CC BY-NC-ND 3.0 US), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subjectChromatin remodeling enzymeen_US
dc.subjectRSCen_US
dc.subjectDNA translocationen_US
dc.subjectEnergy transductionen_US
dc.subjectKinetic modelingen_US
dc.titleKinetic Mechanism of DNA Translocation by the RSC Molecular Motoren_US
dc.typeArticleen_US
kusw.kuauthorFischer, Christopher J.
kusw.kudepartmentPhysics and Astronomyen_US
dc.identifier.doi10.1016/j.abb.2013.01.011en_US
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccess


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This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 3.0 (CC BY-NC-ND 3.0 US), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Except where otherwise noted, this item's license is described as: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 3.0 (CC BY-NC-ND 3.0 US), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.