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    Withaferin A, a Cytotoxic Steroid from Vassobia breWiflora, Induces Apoptosis in Human Head and Neck Squamous Cell Carcinoma

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    Issue Date
    2010-08-20
    Author
    Samadi, Abbas K.
    Tong, Xiaoqin
    Mukerji, Ridhwi
    Zhang, Huaping
    Timmermann, Barbara N.
    Cohen, Mark S.
    Publisher
    Journal of Natural Products
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Rights
    Copyright © 2010 The American Chemical Society and American Society of Pharmacognosy
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    Abstract
    As part of a program to discover drug leads from plant biodiversity, the present investigation was undertaken to explore the anticancer potential of compounds derived from selected Latin American plants. Bioassay-guided fractionation of a crude extract of the aerial parts of Vassobia breviflora led to the isolation of the withanolide-type steroidal lactone withaferin A (1). This compound was tested for antiproliferative activity against the head and neck squamous cell carcinoma (HNSCC) cell lines, MDA1986, JMAR, UM-SCC-2, and JHU011. The inhibitory concentrations to reduce cell viability to 50% (IC50) were determined by the MTS cytotoxicity assay, and 1 reduced cell viability with IC50 values in the range 0.5−2.2 μM. A mechanistic study showed that 1 induces apoptosis and cell death in HNSCC cells as well as a cell-cycle shift from G0/G1 to G2/M. Cells treated with 1 exhibited inactivation of Akt and a reduction in total Akt concentration. This investigation constitutes the first report of the antiproliferative activity of withaferin A (1) against head and neck squamous carcinoma.
    URI
    http://hdl.handle.net/1808/22587
    DOI
    https://doi.org/10.1021/np100112p
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    • Medicinal Chemistry Scholarly Works [225]
    Citation
    Samadi, Abbas K., Xiaoqin Tong, Ridhwi Mukerji, Huaping Zhang, Barbara N. Timmermann, and Mark S. Cohen. "Withaferin A, a Cytotoxic Steroid FromVassobia Breviflora, Induces Apoptosis in Human Head and Neck Squamous Cell Carcinoma." Journal of Natural Products 73.9 (2010): 1476-481.

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    KU Libraries
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    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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