dc.contributor.advisor | Tamerler, Candan | |
dc.contributor.author | VanOosten, Sarah Kay | |
dc.date.accessioned | 2017-01-02T19:39:41Z | |
dc.date.available | 2017-01-02T19:39:41Z | |
dc.date.issued | 2016-08-31 | |
dc.date.submitted | 2016 | |
dc.identifier.other | http://dissertations.umi.com/ku:14883 | |
dc.identifier.uri | http://hdl.handle.net/1808/22333 | |
dc.description.abstract | Silver nanoparticles (AgNPs) are promising candidates for fighting drug-resistant infections because of their intrinsic antimicrobial effect. The antimicrobial efficacy, shown as a result of high-yield design of AgNPs, may inadvertently cause variation in host cells biological responses. While many factors affect AgNP efficacy, their surface is exposed to the biological environment and thus plays a critical role both in preserving antimicrobial efficacy against pathogens, as well as preventing cytotoxicity for host cells. Our approach for controlling nanoparticle surface properties is built upon engineering a biomimetic interface that provides a competitive advantage. Here, we engineered a fusion protein featuring a silver-binding peptide domain (AgBP) to enable self-assembly with green fluorescence protein (GFP) to track assembly. Following AgNP functionalization with GFP-AgBP, their antimicrobial properties were evaluated in conjunction with their cytotoxic properties. GFP-AgBP binding affinity to AgNPs was evaluated using localized surface plasmon resonance. The GFP-AgBP biomimetic interface on AgNP surfaces provided sustained antibacterial efficacy at relatively low concentrations based upon bacterial (Streptococcus mutans) growth inhibition assays. Viability and cytotoxicity measurements in fibroblast cells (NIH/3T3) exposed to protein-functionalized AgNPs showed significant improvement compared to controls. Biointerface engineering offers promise toward tailoring AgNP efficacy while addressing safety concerns to maintain optimum cellular interactions. | |
dc.format.extent | 38 pages | |
dc.language.iso | en | |
dc.publisher | University of Kansas | |
dc.rights | Copyright held by the author. | |
dc.subject | Biomedical engineering | |
dc.subject | antimicrobial | |
dc.subject | biointerface | |
dc.subject | nanoparticles | |
dc.title | Engineered Bio-Silver Nanoparticle Interface Offers Antimicrobial Properties for Improved Cellular Viability | |
dc.type | Thesis | |
dc.contributor.cmtemember | Spencer, Paulette | |
dc.contributor.cmtemember | Snead, Malcolm L | |
dc.thesis.degreeDiscipline | Bioengineering | |
dc.thesis.degreeLevel | M.S. | |
dc.identifier.orcid | | |
dc.rights.accessrights | openAccess | |