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    Wnt Signaling-Mediated Redox Regulation Maintains the Germline Stem Cell Differentiation Niche

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    Wang_ku_0099D_14409_DATA_1.pdf (3.102Mb)
    Issue Date
    2015-12-31
    Author
    Wang, Su
    Publisher
    University of Kansas
    Format
    83 pages
    Type
    Dissertation
    Degree Level
    Ph.D.
    Discipline
    Anatomy & Cell Biology
    Rights
    Copyright held by the author.
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    Abstract
    To remain self-renewal or to differentiate, this is one of the most important question in the stem cell field. Both stem cell maintenance and differentiation are regulated by intrinsic and extrinsic signals from surrounding stromal cells or niche. The Drosophila ovary is a simple but elegant model to study stem cells and their niche. At the tip of Drosophila ovary, a cluster of somatic cap cells form the germline stem cell niche and provide self-renewal signal to maintain GSC self-renewal. When GSC divides, its daughter cell, cystoblast leaves the GSC niche and undergoes differentiation. Outside the self-renewal niche, inner sheath cells are required for germ cell differentiation by functioning as a germ cell differentiation niche. Compared to the self-renewing niche, relatively little is known about the maintenance and function of the differentiation niche. Here we show that the cellular redox state regulated by Wnt signaling is critical for the maintenance and function of the differentiation niche to promote germ cell differentiation. Defective Wnt signaling results in the loss of the differentiation niche, and thus a germ cell differentiation defect. Mechanistically, Wnt signaling controls the expression of multiple glutathione-S-transferase family genes and the cellular redox state. Finally, Wnt2 and Wnt4 function redundantly to maintain active Wnt signaling in the differentiation niche. Therefore, this study has revealed a novel strategy for Wnt signaling in regulating the cellular redox state and maintaining the differentiation niche.
    URI
    http://hdl.handle.net/1808/21930
    Collections
    • Dissertations [4475]
    • KU Med Center Dissertations and Theses [464]

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    KU Libraries
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    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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