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    The Yeast Heat Shock Transcription Factor Changes Conformation in Response to Superoxide and Temperature

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    Issue Date
    2000-05
    Author
    Lee, Sengyong
    Carlson, Tage
    Christian, Noah
    Lea, Kristi
    Raff, Jennifer
    Publisher
    American Society for Cell Biology
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Rights
    Copyright © 2000, The American Society for Cell Biology
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    Abstract
    In vitro DNA-binding assays demonstrate that the heat shock transcription factor (HSF) from the yeast Saccharomyces cerevisiae can adopt an altered conformation when stressed. This conformation, reflected in a change in electrophoretic mobility, requires that two HSF trimers be bound to DNA. Single trimers do not show this change, which appears to represent an alteration in the cooperative interactions between trimers. HSF isolated from stressed cells displays a higher propensity to adopt this altered conformation. Purified HSF can be stimulated in vitro to undergo the conformational change by elevating the temperature or by exposing HSF to superoxide anion. Mutational analysis maps a region critical for this conformational change to the flexible loop between the minimal DNA-binding domain and the flexible linker that joins the DNA-binding domain to the trimerization domain. The significance of these findings is discussed in the context of the induction of the heat shock response by ischemic stroke, hypoxia, and recovery from anoxia, all known to stimulate the production of superoxide.
    URI
    http://hdl.handle.net/1808/21266
    DOI
    https://doi.org/10.1091/mbc.11.5.1753
    Collections
    • Anthropology Scholarly Works [203]
    Citation
    Lee, S., Carlson, T., Christian, N., Lea, K., Kedzie, J., Reilly, J. P., & Bonner, J. J. (2000). The Yeast Heat Shock Transcription Factor Changes Conformation in Response to Superoxide and Temperature. Molecular Biology of the Cell, 11(5), 1753–1764.

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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