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dc.contributor.authorNajarro, Michael A.
dc.contributor.authorHackett, Jennifer L.
dc.contributor.authorSmith, Brittny R.
dc.contributor.authorHighfill, Chad Allen
dc.contributor.authorKing, Elizabeth G.
dc.contributor.authorLong, Anthony D.
dc.contributor.authorMacdonald, Stuart J.
dc.date.accessioned2016-03-29T15:43:46Z
dc.date.available2016-03-29T15:43:46Z
dc.date.issued2015-11
dc.identifier.citationNajarro, Michael A. et al. “Identifying Loci Contributing to Natural Variation in Xenobiotic Resistance in Drosophila.” Ed. Nadia Singh. PLoS Genetics 11.11 (2015): e1005663. PMC. Web. 2 Feb. 2016.en_US
dc.identifier.urihttp://hdl.handle.net/1808/20585
dc.description.abstractNatural populations exhibit a great deal of interindividual genetic variation in the response to toxins, exemplified by the variable clinical efficacy of pharmaceutical drugs in humans, and the evolution of pesticide resistant insects. Such variation can result from several phenomena, including variable metabolic detoxification of the xenobiotic, and differential sensitivity of the molecular target of the toxin. Our goal is to genetically dissect variation in the response to xenobiotics, and characterize naturally-segregating polymorphisms that modulate toxicity. Here, we use the Drosophila Synthetic Population Resource (DSPR), a multiparent advanced intercross panel of recombinant inbred lines, to identify QTL (Quantitative Trait Loci) underlying xenobiotic resistance, and employ caffeine as a model toxic compound. Phenotyping over 1,700 genotypes led to the identification of ten QTL, each explaining 4.5–14.4% of the broad-sense heritability for caffeine resistance. Four QTL harbor members of the cytochrome P450 family of detoxification enzymes, which represent strong a priori candidate genes. The case is especially strong for Cyp12d1, with multiple lines of evidence indicating the gene causally impacts caffeine resistance. Cyp12d1 is implicated by QTL mapped in both panels of DSPR RILs, is significantly upregulated in the presence of caffeine, and RNAi knockdown robustly decreases caffeine tolerance. Furthermore, copy number variation at Cyp12d1 is strongly associated with phenotype in the DSPR, with a trend in the same direction observed in the DGRP (Drosophila Genetic Reference Panel). No additional plausible causative polymorphisms were observed in a full genomewide association study in the DGRP, or in analyses restricted to QTL regions mapped in the DSPR. Just as in human populations, replicating modest-effect, naturally-segregating causative variants in an association study framework in flies will likely require very large sample sizes.en_US
dc.publisherPublic Library of Scienceen_US
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleIdentifying Loci Contributing to Natural Variation in Xenobiotic Resistance in Drosophilaen_US
dc.typeArticleen_US
kusw.kuauthorNajarro, Michael A.
kusw.kuauthorHackett, Jennifer L.
kusw.kuauthorSmith, Brittny R.
kusw.kuauthorHighfill, Chad Allen
kusw.kuauthorMacdonald, Stuart J.
kusw.kudepartmentMolecular Biosciencesen_US
kusw.kudepartmentComputational Biologyen_US
kusw.oastatusfullparticipationen_US
dc.identifier.doi10.1371/journal.pgen.1005663en_US
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccess


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This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Except where otherwise noted, this item's license is described as: This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited