ATTENTION: The software behind KU ScholarWorks is being upgraded to a new version. Starting July 15th, users will not be able to log in to the system, add items, nor make any changes until the new version is in place at the end of July. Searching for articles and opening files will continue to work while the system is being updated.
If you have any questions, please contact Marianne Reed at mreed@ku.edu .
DNA methylation and gene expression in Mimulus guttatus
dc.contributor.author | Colicchio, Jack M. | |
dc.contributor.author | Miura, Fumihito | |
dc.contributor.author | Kelly, John K. | |
dc.contributor.author | Ito, Takashi | |
dc.contributor.author | Hileman, Lena C. | |
dc.date.accessioned | 2016-02-12T21:41:33Z | |
dc.date.available | 2016-02-12T21:41:33Z | |
dc.date.issued | 2015-07-07 | |
dc.identifier.citation | Colicchio, Jack M., Fumihito Miura, John K. Kelly, Takashi Ito, and Lena C. Hileman. "DNA Methylation and Gene Expression in Mimulus Guttatus." BMC Genomics 16.1 (2015): n. pag. doi:10.1186/s12864-015-1668-0. | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/20063 | |
dc.description | A grant from the One-University Open Access Fund at the University of Kansas was used to defray the author's publication fees in this Open Access journal. The Open Access Fund, administered by librarians from the KU, KU Law, and KUMC libraries, is made possible by contributions from the offices of KU Provost, KU Vice Chancellor for Research & Graduate Studies, and KUMC Vice Chancellor for Research. For more information about the Open Access Fund, please see http://library.kumc.edu/authors-fund.xml. | |
dc.description.abstract | BackgroundThe presence of methyl groups on cytosine nucleotides across an organism’s genome (methylation) is a major regulator of genome stability, crossing over, and gene regulation. The capacity for DNA methylation to be altered by environmental conditions, and potentially passed between generations, makes it a prime candidate for transgenerational epigenetic inheritance. Here we conduct the first analysis of the Mimulus guttatus methylome, with a focus on the relationship between DNA methylation and gene expression.ResultsWe present a whole genome methylome for the inbred line Iron Mountain 62 (IM62). DNA methylation varies across chromosomes, genomic regions, and genes. We develop a model that predicts gene expression based on DNA methylation (R2 = 0.2). Post hoc analysis of this model confirms prior relationships, and identifies novel relationships between methylation and gene expression. Additionally, we find that DNA methylation is significantly depleted near gene transcriptional start sites, which may explain the recently discovered elevated rate of recombination in these same regions.ConclusionsThe establishment here of a reference methylome will be a useful resource for the continued advancement of M. guttatus as a model system. Using a model-based approach, we demonstrate that methylation patterns are an important predictor of variation in gene expression. This model provides a novel approach for differential methylation analysis that generates distinct and testable hypotheses regarding gene expression. | en_US |
dc.publisher | BioMed Central | en_US |
dc.rights | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0 | |
dc.title | DNA methylation and gene expression in Mimulus guttatus | en_US |
dc.type | Article | |
kusw.kuauthor | Kelly, John K. | |
kusw.kudepartment | Ecology & Evolutionary Biology | en_US |
dc.identifier.doi | 10.1186/s12864-015-1668-0 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item meets KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's license is described as: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.