Tumor suppressive microRNA-137 negatively regulates Musashi-1 and colorectal cancer progression
dc.contributor.author | Smith, Amber Rae | |
dc.contributor.author | Marquez, Rebecca T. | |
dc.contributor.author | Tsao, Wei-Chung | |
dc.contributor.author | Pathak, Surajit | |
dc.contributor.author | Roy, Alexandria | |
dc.contributor.author | Ping, Jie | |
dc.contributor.author | Wilkerson, Bailey | |
dc.contributor.author | Lan, Lan | |
dc.contributor.author | Meng, Wenjian | |
dc.contributor.author | Neufeld, Kristi L. | |
dc.contributor.author | Sun, Xiao-Feng | |
dc.contributor.author | Xu, Liang | |
dc.date.accessioned | 2016-02-12T21:40:27Z | |
dc.date.available | 2016-02-12T21:40:27Z | |
dc.date.issued | 2015-03-30 | |
dc.identifier.citation | Smith, Amber R., Rebecca T. Marquez, Wei-Chung Tsao, Surajit Pathak, Alexandria Roy, Jie Ping, Bailey Wilkerson, Lan Lan, Wenjian Meng, Kristi L. Neufeld, Xiao-Feng Sun, and Liang Xu. "Tumor Suppressive MicroRNA-137 Negatively Regulates Musashi-1 and Colorectal Cancer Progression." Oncotarget 6.14 (2015): 12558-2573. doi:10.18632/oncotarget.3726. | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/20058 | |
dc.description.abstract | Stem cell marker, Musashi-1 (MSI1) is over-expressed in many cancer types; however the molecular mechanisms involved in MSI1 over-expression are not well understood. We investigated the microRNA (miRNA) regulation of MSI1 and the implications this regulation plays in colorectal cancer. MicroRNA miR-137 was identified as a MSI1-targeting microRNA by immunoblotting and luciferase reporter assays. MSI1 protein was found to be highly expressed in 79% of primary rectal tumors (n=146), while miR-137 expression was decreased in 84% of the rectal tumor tissues (n=68) compared to paired normal mucosal samples. In addition to reduced MSI1 protein, exogenous expression of miR-137 inhibited cell growth, colony formation, and tumorsphere growth of colon cancer cells. Finally, in vivo studies demonstrated that induction of miR-137 can decrease growth of human colon cancer xenografts. Our results demonstrate that miR-137 acts as a tumor-suppressive miRNA in colorectal cancers and negatively regulates oncogenic MSI1. | en_US |
dc.publisher | Impact Journals | en_US |
dc.rights | This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Tumor-initiating cells | en_US |
dc.subject | MicroRNAs | en_US |
dc.subject | RNA-binding proteins | en_US |
dc.subject | Colon cancer | en_US |
dc.subject | Rectal cancer | en_US |
dc.title | Tumor suppressive microRNA-137 negatively regulates Musashi-1 and colorectal cancer progression | en_US |
dc.type | Article | |
kusw.kuauthor | Marquez, Rebecca T. | |
kusw.kudepartment | Higuchi Biosciences Center | en_US |
dc.identifier.doi | 10.18632/oncotarget.3726 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item meets KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess |
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