A mitochondrial DNA restriction enzyme cleavage map for the scorpion Hadrurus arizonensis (Iuridae).

Abstract

Mitochondrial DNA (mtDNA) was prepared from a single individual of the scorpion Hadrurus arizonensis Ewing. The total size of the mitochondrial genome was estimated to be 13 850 to 14 000 base pairs. The mtDNA was surveyed for cleavage sites using 17 six-base restriction enzymes and three four-base enzymes. The technique of double digests was used to construct a map of the cleavage sites generated in this mtDNA by nine six-base restriction enzymes.