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    Revealing Remodeler Function: Varied and Unique

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    Eastlund_ku_0099D_14072_DATA_1.pdf (2.694Mb)
    Issue Date
    2015-05-31
    Author
    Eastlund, Allen
    Publisher
    University of Kansas
    Format
    107 pages
    Type
    Dissertation
    Degree Level
    Ph.D.
    Discipline
    Physics & Astronomy
    Rights
    Copyright held by the author.
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    Abstract
    Chromatin remodelers perform a necessary and required function for the successful expression of our genetic code. By modifying, shifting, or ejecting nucleosomes from the chromatin structure they allow access to the underlying DNA to the rest of the cell’s machinery. This research has focused on two major remodeler motors from major families of chromatin remodelers: the trimeric motor domain of RSC and the motor domain of the ISWI family, ISWI. Using primarily stopped-flow spectrofluorometry, I have categorized the time-dependent motions of these motor domains along their preferred substrate, double-stranded DNA. Combined with collected ATP utilization data, I present the subsequent analysis and associated conclusions that stem from the underlying assumptions and models. Interestingly, there is little in common between the investigated proteins aside from their favored medium. While RSC exhibits modest translocation characteristics and highly effective motion with the ability for large molecular forces, ISWI is not only structurally different but highly inefficient in its motion leading to difficulties in determining its specific translocation mechanics. While chromatin remodeling is a ubiquitous facet of eukaryotic life, there remains much to be understood about their general mechanisms.
    URI
    http://hdl.handle.net/1808/19031
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    • Dissertations [4660]
    • Physics & Astronomy Dissertations and Theses [121]

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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