Show simple item record

dc.contributor.authorMagee, P. T.
dc.contributor.authorBowdin, Linda
dc.contributor.authorStaudinger, Jeffrey Leonard
dc.date.accessioned2015-05-27T16:29:56Z
dc.date.available2015-05-27T16:29:56Z
dc.date.issued1992-10
dc.identifier.citationMagee, P. T., L. Bowdin, and Jeffrey Leonard Staudinger. "Comparison of Molecular Typing Methods for Candida Albicans." Journal of Clinical Microbiology 30.10 (1992): 2674-679. Web. 27 May 2015.en_US
dc.identifier.urihttp://hdl.handle.net/1808/17848
dc.descriptionThis is the published version. Copyright 1992 American Society for Microbiology.en_US
dc.description.abstractFour molecular approaches to determining the types of Candida albicans strains were compared. The strains used were those whose repeated DNA (ribosomal and mitochondrial) EcoRI restriction fragment length polymorphisms (RFLP) were determined by Stevens et al. (D. A. Stevens, F. C. Odds, and S. Scherer, Rev. Infect. Dis. 12:258-266, 1990). Scherer and Stevens (S. Scherer and D. A. Stevens, Proc. Natl. Acad. Sci. USA 85:1452-1456, 1988) used the same strains to examine the Southern blots of genomic EcoRI digests probed with the repeated sequence 27A. The results of these investigators were compared with determinations of RFLPs generated from repeated DNA by the enzyme HinfI and examination of the karyotypes of strains under two sets of conditions, one for the smaller chromosomes and one for the larger ones. Analysis of RFLPs of repeated DNA is most convenient but shows the lowest degree of resolution. Use of the repeated sequence and use of karyotype have very high resolution, but the former method is more convenient than the latter. HinfI digestion is more sensitive than EcoRI digestion but equally convenient. By using all four methods, separate types were identified for 18 of the 20 strains examined.en_US
dc.publisherAmerican Society for Microbiologyen_US
dc.titleComparison of molecular typing methods for Candida albicans.en_US
dc.typeArticle
kusw.kuauthorStaudinger, Jeffrey Leonard
kusw.kudepartmentPharmacology & Toxicologyen_US
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item does not meet KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record