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    Comparative immunogenicity in rhesus monkeys of DNA plasmid, recombinant vaccinia virus, and replication-defective adenovirus vectors expressing a human immunodeficiency virus type 1 gag gene

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    VolkinD_JV_77(11)6305.pdf (270.2Kb)
    Issue Date
    2003-06-01
    Author
    Volkin, David B.
    Casimiro, Danilo R.
    Chen, Ling
    Fu, Tong-Ming
    Evans, Robert K.
    Caulfield, Michael J.
    Davies, Mary-Ellen
    Tang, Aimin
    Chen, Minchun
    Huang, Lingyi
    Harris, Virginia
    Freed, Daniel C.
    Wilson, Keith A.
    Dubey, Sheri
    Zhu, De-Min
    Nawrocki, Denise K.
    Mach, Henryk
    Troutman, Robert D.
    Isopi, Lynne
    Williams, Donna
    Hurni, William
    Xu, Zheng
    Smith, Jeffrey G.
    Wang, Su
    Liu, Xu
    Guan, Liming
    Long, Romnie
    Trigona, Wendy
    Heidecker, Gwendolyn J.
    Perry, Helen C.
    Persaud, Natasha
    Toner, Timothy J.
    Su, Qin
    Liang, Xiaoping
    Youil, Rima
    Chastain, Michael
    Bett, Andrew J.
    Emini, Emilio A.
    Shiver, John W.
    Publisher
    American Society for Microbiology
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Metadata
    Show full item record
    Abstract
    Cellular immune responses, particularly those associated with CD3+ CD8+ cytotoxic T lymphocytes (CTL), play a primary role in controlling viral infection, including persistent infection with human immunodeficiency virus type 1 (HIV-1). Accordingly, recent HIV-1 vaccine research efforts have focused on establishing the optimal means of eliciting such antiviral CTL immune responses. We evaluated several DNA vaccine formulations, a modified vaccinia virus Ankara vector, and a replication-defective adenovirus serotype 5 (Ad5) vector, each expressing the same codon-optimized HIV-1 gag gene for immunogenicity in rhesus monkeys. The DNA vaccines were formulated with and without one of two chemical adjuvants (aluminum phosphate and CRL1005). The Ad5-gag vector was the most effective in eliciting anti-Gag CTL. The vaccine produced both CD4+ and CD8+ T-cell responses, with the latter consistently being the dominant component. To determine the effect of existing antiadenovirus immunity on Ad5-gag-induced immune responses, monkeys were exposed to adenovirus subtype 5 that did not encode antigen prior to immunization with Ad5-gag. The resulting anti-Gag T-cell responses were attenuated but not abolished. Regimens that involved priming with different DNA vaccine formulations followed by boosting with the adenovirus vector were also compared. Of the formulations tested, the DNA-CRL1005 vaccine primed T-cell responses most effectively and provided the best overall immune responses after boosting with Ad5-gag. These results are suggestive of an immunization strategy for humans that are centered on use of the adenovirus vector and in which existing adenovirus immunity may be overcome by combined immunization with adjuvanted DNA and adenovirus vector boosting.
    Description
    This is the publisher's version, also available electronically from "http://jvi.asm.org".
    URI
    http://hdl.handle.net/1808/17716
    DOI
    https://doi.org/10.1128/JVI.77.11.6305-6313.2003
    Collections
    • Pharmacy Scholarly Works [293]
    Citation
    Volkin, D. B., et al. (2003) Comparative immunogenicity in rhesus monkeys of DNA plasmid, recombinant vaccinia virus, and replication-defective adenovirus vectors expressing a human immunodeficiency virus type 1 gag gene. Journal of Virology, 77(11), 6305-6313. http://www.dx.doi.org/10.1128/JVI.77.11.6305-6313.2003

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    KU Libraries
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    785-864-8983

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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