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    Cytoplasmic dynein’s mitotic spindle pole localization requires a functional anaphase-promoting complex, γ-tubulin, and NUDF/LIS1 in Aspergillus nidulans

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    Oakley_MBC_16(8)3591.pdf (670.7Kb)
    Issue Date
    2005-08-01
    Author
    Li, Shihe
    Oakley, C. Elizabeth
    Chen, Guifang
    Han, Xiaoyan
    Oakley, Berl R.
    Xiang, Xin
    Publisher
    American Society for Cell Biology
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
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    Abstract
    In Aspergillus nidulans, cytoplasmic dynein and NUDF/LIS1 are found at the spindle poles during mitosis, but they seem to be targeted to this location via different mechanisms. The spindle pole localization of cytoplasmic dynein requires the function of the anaphase-promoting complex (APC), whereas that of NUDF does not. Moreover, although NUDF's localization to the spindle poles does not require a fully functional dynein motor, the function of NUDF is important for cytoplasmic dynein's targeting to the spindle poles. Interestingly, a γ-tubulin mutation, mipAR63, nearly eliminates the localization of cytoplasmic dynein to the spindle poles, but it has no apparent effect on NUDF's spindle pole localization. Live cell analysis of the mipAR63 mutant revealed a defect in chromosome separation accompanied by unscheduled spindle elongation before the completion of anaphase A, suggesting that γ-tubulin may recruit regulatory proteins to the spindle poles for mitotic progression. In A. nidulans, dynein is not apparently required for mitotic progression. In the presence of a low amount of benomyl, a microtubule-depolymerizing agent, however, a dynein mutant diploid strain exhibits a more pronounced chromosome loss phenotype than the control, indicating that cytoplasmic dynein plays a role in chromosome segregation.
    Description
    This is the publisher's version, also available electronically from "http://www.molbiolcell.org".
    URI
    http://hdl.handle.net/1808/17526
    DOI
    https://doi.org/10.1091/mbc.E04-12-1071
    Collections
    • Molecular Biosciences Scholarly Works [594]
    Citation
    Li, S., Oakley, C., Chen, G., Han, X., Oakley, B., & Xiang, X. (2005). Cytoplasmic dynein’s mitotic spindle pole localization requires a functional anaphase-promoting complex, γ-tubulin, and NUDF/LIS1 in Aspergillus nidulans. Moleculary Biology of the Cell, 16(8), 3591-3605. http://www.dx.doi.org/10.1091/mbc.E04-12-1071

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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