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    Structure of the Yersinia pestis tip protein LcrV refined to 1.65 A resolution

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    DeGuzmanR_May2013.pdf (780.1Kb)
    Issue Date
    2013-05-01
    Author
    Chaudhury, Sukanya
    Battaile, Kevin P.
    Lovell, Scott
    Plano, Gregory V.
    De Guzman, Roberto N.
    Publisher
    International Union of Crystallography
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
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    Abstract
    The human pathogen Yersinia pestis requires the assembly of the type III secretion system (T3SS) for virulence. The structural component of the T3SS contains an external needle and a tip complex, which is formed by LcrV in Y. pestis. The structure of an LcrV triple mutant (K40A/D41A/K42A) in a C273S background has previously been reported to 2.2 Å resolution. Here, the crystal structure of LcrV without the triple mutation in a C273S background is reported at a higher resolution of 1.65 Å. Overall the two structures are similar, but there are also notable differences, particularly near the site of the triple mutation. The refined structure revealed a slight shift in the backbone positions of residues Gly28-Asn43 and displayed electron density in the loop region consisting of residues Ile46-Val63, which was disordered in the original structure. In addition, the helical turn region spanning residues Tyr77-Gln95 adopts a different orientation.
    Description
    This is the publisher's version, also available electronically from http://scripts.iucr.org/cgi-bin/paper?S1744309113008579.
    URI
    http://hdl.handle.net/1808/16998
    DOI
    https://doi.org/10.1107/S1744309113008579
    ISSN
    1744-3091
    Collections
    • Molecular Biosciences Scholarly Works [590]
    Citation
    Chaudhury, S.; Battaile, K. P.; Lovell, S.; Plano, G. V.; De Guzman, Roberto N. (2013). "Structure of the Yersinia pestis tip protein LcrV refined to 1.65 A resolution." Acta Crystallographica Section F., F69:477-481. http://www.dx.doi.org/10.1107/S1744309113008579.

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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