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    The Home Microbiome and Childhood Asthma

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    Ciaccio_ku_0099M_13265_DATA_1.pdf (979.3Kb)
    Issue Date
    2014-05-31
    Author
    Ciaccio, Christina Elizabeth
    Publisher
    University of Kansas
    Format
    25 pages
    Type
    Thesis
    Degree Level
    M.S.
    Discipline
    Preventive Medicine and Public Health
    Rights
    This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
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    Abstract
    BACKGROUND: Exposure to microorganisms has repeatedly been found to influence the development of atopic diseases, such as asthma. This relationship has been best understood as an inverse correlation between microbial diversity and atopic disease. Innovative techniques have recently been developed that can more comprehensively characterize microbial communities. OBJECTIVE: To characterize the home microbiota of asthmatics and non-asthmatics utilizing 16S rRNA based phylogenetic analysis by microarray technology METHODS: This cross-sectional study utilized dust samples collected as part of the Kansas City Safe and Healthy Homes Program. DNA was extracted from home dust and bacterial 16S rRNA genes amplified. Bacterial products were hybridized to the PhyloChip Array and scanned using a GeneArray scanner. The Adonis test was used to determine significant differences in the whole microbiome. Welch's t-test was used to determine significant abundance differences and genus-level richness differences. RESULTS: 1741 operational taxonomic units (OTUs) were found in at least one sample. Bacterial genus richness did not differ in the homes of asthmatics and non-asthmatics (p=0.09). The microbial profile was significantly different between the two groups of homes (p=0.025). All of the top 12 OTUs with significant abundance differences were increased in homes of asthmatic children and belonged to one of the five phyla (p=0.001 to p=7.2 x 10-6). Nearly half of significant abundance differences belonged to the phylum Cyanobacteria or Proteobacteria. CONCLUSIONS: These results suggest that home dust has a characteristic microbiota which is disturbed in the homes of asthmatics, resulting in a particular abundance of Cyanobacteria and Proteobacteria. Further investigations are needed which utilize high throughput technology to further clarify how home microbial exposures influence human health and disease.
    URI
    http://hdl.handle.net/1808/14498
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    • KU Med Center Dissertations and Theses [464]
    • Theses [3908]

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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