Assessments of Biodiversity Based on Molecular Markers and Morphological Traits among West-Bank, Palestine Fig Genotypes (Ficus carica L.)

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Issue Date
2012-09-26Author
Basheer-Salimia, Rezq
Awad, Murad
Ward, Joy K.
Publisher
Scientific Research
Type
Article
Article Version
Scholarly/refereed, publisher version
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Show full item recordAbstract
Both morphological characters and PCR-based RAPD approaches were used to determine the genetic diversity and re- latedness among nine fig genotypes grown at the northern region of the West-Bank, Palestine. Although we tested 28 primers for the RAPD technique, only 9 produced reasonable amplification products. A total of 57 DNA loci were de- tected in which 70.2% were polymorphic. DNA fragments presented a minimum of 3 and a maximum of 9 polymorphic bands using primers OPT-10 and OPA-18, respectively. Primers exhibited collective resolving power values (Rp) of 18.826. The Mwazi genotype showed the highest genetic distances among all of the other genotypes. Morphologically, considerable variations were found using 41 quantitative and qualitative traits. Adloni could be a very promising geno- type for fresh consumption due to its very late maturation period, extended harvesting period, variable fruit size, and easy skin peeling. In addition, 7 genotypes presented firm fruits, which are a very important criterion for exporting purposes. Dendrogram constructed by UPGMA based on RAPD banding patterns appear somewhat contradictory to the morphological descriptors particularly with Swadi and Biadi genotypes (closed genetically and distanced morphologi- cally), which might be attributed to the phenotypic modifications caused by environmental differences across regions. These preliminary results will make a fundamental contribution to further genetic improvement of fig crops for the region.
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This is the publisher's version, also available electronically from http://www.scirp.org/journal/ajps/
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Citation
Rezq Basheer-Salimia, Murad Awad, and Joy Ward. "Assessments of Biodiversity Based on Molecular Markers and Morphological Traits among West-Bank, Palestine Fig Genotypes (Ficus carica L.)." American Journal of Plant Sciences 3:1241-1251. http://dx.doi.org/10.4236/ajps.2012.39150
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