Potent Adjuvanticity of a Pure TLR7-Agonistic Imidazoquinoline Dendrimer
dc.contributor.author | Shukla, Nikunj M. | |
dc.contributor.author | Salunke, Deepak B. | |
dc.contributor.author | Balakrishna, Rajalakshmi | |
dc.contributor.author | Mutz, Cole A. | |
dc.contributor.author | Malladi, Subbalakshmi S. | |
dc.contributor.author | David, Sunil A. | |
dc.date.accessioned | 2014-03-19T21:41:21Z | |
dc.date.available | 2014-03-19T21:41:21Z | |
dc.date.issued | 2012-08-28 | |
dc.identifier.citation | Shukla, N. M., Salunke, D. B., Balakrishna, R., Mutz, C. A., Malladi, S. S., & David, S. A. (2012). Potent Adjuvanticity of a Pure TLR7-Agonistic Imidazoquinoline Dendrimer. PLoS ONE, 7(8). http://dx.doi.org/10.1371/journal.pone.0043612 | |
dc.identifier.uri | http://hdl.handle.net/1808/13280 | |
dc.description.abstract | Engagement of toll-like receptors (TLRs) serve to link innate immune responses with adaptive immunity and can be exploited as powerful vaccine adjuvants for eliciting both primary and anamnestic immune responses. TLR7 agonists are highly immunostimulatory without inducing dominant proinflammatory cytokine responses. We synthesized a dendrimeric molecule bearing six units of a potent TLR7/TLR8 dual-agonistic imidazoquinoline to explore if multimerization of TLR7/8 would result in altered activity profiles. A complete loss of TLR8-stimulatory activity with selective retention of the TLR7-agonistic activity was observed in the dendrimer. This was reflected by a complete absence of TLR8-driven proinflammatory cytokine and interferon (IFN)-γ induction in human PBMCs, with preservation of TLR7-driven IFN-α induction. The dendrimer was found to be superior to the imidazoquinoline monomer in inducing high titers of high-affinity antibodies to bovine α-lactalbumin. Additionally, epitope mapping experiments showed that the dendrimer induced immunoreactivity to more contiguous peptide epitopes along the amino acid sequence of the model antigen. | |
dc.description.sponsorship | This work was supported by National Institutes of Health/National Institute of Allergy and Infectious Diseases contract HHSN272200900033C. | |
dc.publisher | Public Library of Science | |
dc.rights | © Shukla et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Anitbodies | |
dc.subject | Bovines | |
dc.subject | Cytokines | |
dc.subject | Enzyme-linked immunoassays | |
dc.subject | Epitope mapping | |
dc.subject | Immune response | |
dc.subject | Peptide mapping | |
dc.subject | Protein sequencing | |
dc.title | Potent Adjuvanticity of a Pure TLR7-Agonistic Imidazoquinoline Dendrimer | |
dc.type | Article | |
kusw.kuauthor | Shukla, Nikunj M. | |
kusw.kuauthor | Salunke, Deepak B. | |
kusw.kuauthor | Balakrishna, Rajalakshmi | |
kusw.kuauthor | Mutz, Cole A. | |
kusw.kuauthor | Malladi, Subbalakshmi S. | |
kusw.kuauthor | David, Sunil A. | |
kusw.kudepartment | Department of Medical Chemistry | |
kusw.oastatus | fullparticipation | |
dc.identifier.doi | 10.1371/journal.pone.0043612 | |
dc.identifier.orcid | https://orcid.org/0000-0002-1241-9146 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item meets KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess |
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Except where otherwise noted, this item's license is described as: © Shukla et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.