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    Decellularized cartilage as a chondroinductive material for cartilage tissue engineering

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    Renth_ku_0099M_13135_DATA_1.pdf (1.479Mb)
    Issue Date
    2013-12-31
    Author
    Renth, Amanda
    Publisher
    University of Kansas
    Format
    99 pages
    Type
    Thesis
    Degree Level
    M.S.
    Discipline
    Bioengineering
    Rights
    This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
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    Abstract
    Cartilage defects, whether caused by osteoarthritis, joint trauma, or other disease, have provoked a wide variety of tissue engineering scaffold strategies in recent years. Traditionally, cartilage tissue engineering scaffolds have utilized synthetic polymer components to form hydrogels or other porous matrices. However, components found within the extracellular matrix (ECM) such as collagen, glycosaminoglycans (GAGs), and ECM-based matrices have emerged as an essential subset of biomaterials for tissue engineering scaffolds. The objective of this research was to develop and evaluate decellularized cartilage (DCC) as a chondroinductive material for cartilage tissue engineering applications. This work was successful in developing a decellularization method for hyaline cartilage fragments that removed 99% of cells, while retaining 87% of GAGs and also in determining a method to produce a homogenous nanopowder of DCC. Additionally, this research was the first to examine the ability of DCC to induce chondrogenesis in stem cells by quantifying gene expression of chondrogenic markers. The results demonstrate for the first time that DCC can indeed upregulate chondrogenic markers and may be a new chondroinductive material that can provide microenvironmental cues and signaling to promote stem cell differentiation in cartilage regeneration.
    URI
    http://hdl.handle.net/1808/12991
    Collections
    • Engineering Dissertations and Theses [1055]
    • Theses [3827]

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    KU Libraries
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    785-864-8983

    KU Libraries
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    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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