KU ScholarWorks

  • myKU
  • Email
  • Enroll & Pay
  • KU Directory
    • Login
    View Item 
    •   KU ScholarWorks
    • Dissertations and Theses
    • Theses
    • View Item
    •   KU ScholarWorks
    • Dissertations and Theses
    • Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Synthesis and Characterization of MOG-IDAC and PLP-PEG-B7AP Molecules for Efficacy Evaluation in the EAE mouse model

    Thumbnail
    View/Open
    Stewart_ku_0099M_12843_DATA_1.pdf (1.648Mb)
    Issue Date
    2012-05-31
    Author
    Stewart, John M.
    Publisher
    University of Kansas
    Format
    71 pages
    Type
    Thesis
    Degree Level
    M.S.
    Discipline
    Pharmaceutical Chemistry
    Rights
    This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
    Metadata
    Show full item record
    Abstract
    Synthesis, Formulation, and In vivo Evaluation of MOG-PEG-IDAC and PLP-PEG-B7AP for Targeting APC to Suppress EAE John M. Stewart, Crisandra Wilkie, Barlas Buyuktimkin, Ahmed Badawi, Paul Kiptoo, and Teruna J. Siahaan Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence KS, 66047 There are two long-term objective of this project 1. Is to use a novel I-Domain Antigen Conjugate (IDAC) molecule to target antigenic peptide to APC to control autoimmune diseases. The short-term objectives of this project are to synthesize, formulate, and evaluate the efficacy of Myelin Oligodendrocyte Glycoprotein (MOG) MOG-PEG-IDAC molecules in suppressing Experimental Autoimmune Encephalomyelitis (EAE) in animal models. The MOG-PEG-IDAC molecule is hypothesized to simultaneously bind to major histocompatibility complex II (MHC-II) and intercellular adhesion molecule 1 (ICAM-1) on the surface of antigen-presenting cells (APC). 2. Is to evaluate the efficacy of PLP-PEG-B7AP in suppressing EAE in mice. The PLP-PEG-B7AP molecule is hypothesized to simultaneously bind to the MHC-II and B7 molecule on the surface of the APC. This binding blocks the formation of the "immunological synapse" and will generate the regulatory response to suppress EAE. Two specific aims are proposed to carry out the short-term objectives. The first specific aim is to design MOG-PEG-IDAC molecules. The second specific aim is to evaluate the efficacy of PLP-PEG-B7AP in suppressing EAE in animal models. To date, the synthesis and characterization of the MOG-PEG-IDAC has been completed, along with EAE animal studies of the PLP-PEG-B7AP.
    URI
    http://hdl.handle.net/1808/12189
    Collections
    • Theses [3228]
    • Pharmaceutical Chemistry Dissertations and Theses [90]

    Items in KU ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.


    We want to hear from you! Please share your stories about how Open Access to this item benefits YOU.


    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    Browse

    All of KU ScholarWorksCommunities & CollectionsThis Collection

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

      Contact KU ScholarWorks
    Lawrence, KS | Maps
     
    • Academics
    • Admission
    • Alumni
    • Athletics
    • Campuses
    • Giving
    • Jobs

    The University of Kansas prohibits discrimination on the basis of race, color, ethnicity, religion, sex, national origin, age, ancestry, disability, status as a veteran, sexual orientation, marital status, parental status, gender identity, gender expression and genetic information in the University’s programs and activities. The following person has been designated to handle inquiries regarding the non-discrimination policies: Director of the Office of Institutional Opportunity and Access, IOA@ku.edu, 1246 W. Campus Road, Room 153A, Lawrence, KS, 66045, (785)864-6414, 711 TTY.

     Contact KU
    Lawrence, KS | Maps