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    THE ENTEROHEMORRHAGIC ESCHERICHIA COLI EFFECTOR PROTEIN NLEF BINDS MAMMALIAN HOST PROTEINS

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    Olsen_ku_0099M_12491_DATA_1.pdf (17.19Mb)
    Issue Date
    2012-12-31
    Author
    Olsen, Rachel Lee
    Publisher
    University of Kansas
    Format
    82 pages
    Type
    Thesis
    Degree Level
    M.A.
    Discipline
    Microbiology, Molecular Genetics & Immunology
    Rights
    This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
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    Abstract
    The extracellular human pathogens enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) and the related mouse pathogen Citrobacter rodentium inject type III secretion system (T3SS) effector proteins to promote their replication, survival and transmission. The mechanisms of action and the host targets of T3SS effectors are under active investigation because of their importance to bacterial virulence. The non-locus of enterocyte effacement (LEE)-encoded protein F, NleF, contributes to E. coli and Citrobacter colonization of animals through an unclear mechanism. Here we sought to characterize the host binding partners of NleF. Using a yeast two-hybrid screen, we identified a set of mammalian proteins as NleF-binding partners including Tmp21, a type-I integral membrane protein and COPI-vesicle receptor involved in trans-Golgi network function. We confirmed this interaction using bacterial two-hybrid, immunoprecipitation and bimolecular fluorescence complementation (BiFC). To consider the effects of NleF on protein trafficking, we expressed a temperature-sensitive vesicular stomatitis virus glycoprotein (VSVG) with temperature dependent localization and monitored protein trafficking. We determined that NleF does not block, but rather slows the intracellular trafficking of VSVG from endoplasmic reticulum to Golgi.
    URI
    http://hdl.handle.net/1808/11450
    Collections
    • Molecular Biosciences Dissertations and Theses [270]
    • Theses [3828]

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    KU Libraries
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    785-864-8983

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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