Dopamine Release and Uptake in Animal Models of Neurological Diseases
Issue Date
2011-5-31Author
Fulks, Jenny Lynn
Publisher
University of Kansas
Format
144 pages
Type
Dissertation
Degree Level
Ph.D.
Discipline
Chemistry
Rights
This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
Metadata
Show full item recordAbstract
Fast scan cyclic voltammetry (FSCV) can be utilized to detect neurotransmitter release and uptake in brain slices. When combining this technique with disease state animals models, information for therapeutic targets can be obtained. Herein, the evaluation of stimulated dopamine release and uptake in animal models was used in conjunction with pharmacological agents to assess neurological problems caused by Fragile X syndrome and chemotherapy. Fragile X Syndrome, a genetic form of mental retardation caused by a trinucleotide repeat expansion, was investigated by combining behavioral analysis and FSCV to determine alterations in dopamine release, dopamine uptake, effects of amphetamine treatments, and the functionality of the D2 autoreceptor. Side effects of chemotherapy can include alteration in neurological function and were investigated by using FSCV to monitor for effect on dopamine release and uptake patterns in the striatum of rats being dosed with Carboplatin, a chemotherapeutic drug. Finally, instrumentation was established to use caged compounds to photo release glutamate while collecting FSCV data of dopamine release to evaluate instantaneous effects of glutamate on the dopamine system in brain slices.
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- Chemistry Dissertations and Theses [335]
- Dissertations [4625]
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