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dc.contributor.advisorDesaire, Heather
dc.contributor.authorToumi, Melinda L.
dc.date.accessioned2012-11-19T23:41:38Z
dc.date.available2012-11-19T23:41:38Z
dc.date.issued2010-05-31
dc.date.submitted2010
dc.identifier.otherhttp://dissertations.umi.com/ku:10928
dc.identifier.urihttp://hdl.handle.net/1808/10407
dc.description.abstractProteins and glycoproteins have the potential to improve health when used as therapeutic products for prevention or treatment of disease, but methods are needed for solving some remaining challenges to production, optimization, and analysis. In this dissertation, novel glycosylation engineering strategies are used to solve challenges in conventional protein production and are used to improve therapeutic protein stability by blocking asparagine deamidation, a ubiquitous cause of protein degradation. Glycan structures on therapeutic glycoproteins must be optimized to avoid negative impacts on pharmacological properties. Methods to optimize glycans are described within, using novel, extracellular glycan trimming reactions performed with glycosidases that can be implemented without harm to protein activity or stability. Finally, analysis of proteins with mass spectrometric peptide mass fingerprinting techniques can be complex, so mass defect filters used for data analysis were improved by determining the correct filter size, using experimental human protein data.
dc.format.extent156 pages
dc.language.isoEN
dc.publisherUniversity of Kansas
dc.rightsThis item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
dc.subjectAnalytical chemistry
dc.subjectPharmaceutical chemistry
dc.subjectBiopharmaceutics
dc.subjectGlycobiology
dc.subjectGlycoprotein
dc.subjectMass spectrometry
dc.subjectProtein
dc.titleTherapeutic Protein and Glycoprotein Production, Optimization, and Analysis Methods
dc.typeDissertation
dc.contributor.cmtememberLunte, Susan M.
dc.contributor.cmtememberWeis, David
dc.contributor.cmtememberJackson, Timothy A.
dc.contributor.cmtememberStaudinger, Jeffrey L
dc.thesis.degreeDisciplineChemistry
dc.thesis.degreeLevelPh.D.
kusw.oastatusna
kusw.oapolicyThis item does not meet KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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