KUKU

KU ScholarWorks

  • myKU
  • Email
  • Enroll & Pay
  • KU Directory
    • Login
    View Item 
    •   KU ScholarWorks
    • Dissertations and Theses
    • Theses
    • View Item
    •   KU ScholarWorks
    • Dissertations and Theses
    • Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    MICRO-RNA146B PROMOTES ALVEOLAR PROGENITOR CELL MAINTENANCE THROUGH PREFERENTIAL REGULATION OF STAT3B

    Thumbnail
    View/Open
    Elsarraj_ku_0099M_12323_DATA_1.pdf (6.503Mb)
    Issue Date
    2012-08-31
    Author
    Elsarraj, Hanan Sataa
    Publisher
    University of Kansas
    Format
    66 pages
    Type
    Thesis
    Degree Level
    M.A.
    Discipline
    Pathology & Laboratory Medicine
    Rights
    This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
    Metadata
    Show full item record
    Abstract
    In this research, we have demonstrated that miRNA146b-5p (miR146b) is a hormonally regulated miRNA that participates in the maintenance of mammary alveolar progenitors during pregnancy and lactation by preferential regulation of STAT3B. Recently, our laboratory isolated distinct ductal, alveolar and multipotent mammary progenitors by single-cell cloning of the COMMA-D (CD) cell line. Molecular characterization of the distinct clones showed significantly higher expression of miR146b in the CD derived alveolar compared to the ductal and multipotent progenitors. Additionally, there was a significant rise in miR146b mRNA levels in the pregnant and lactating mouse mammary glands and in virgin glands following in vivo administration of estrogen and progesterone. An analysis of mammary epithelial subpopulations during pregnancy showed a significantly higher expression of miR146b in the alveolar luminal progenitors compared to stem and differentiated luminal cells. Induced knockdown of miR146b led to increased cell death in the CD derived alveolar progenitors and a significant reduction in the percentage of primary mouse mammary alveolar luminal progenitors. Among several of the candidate targets, miR146b regulated STAT3B and to a lesser extent STAT3A. A reporter construct using STAT3A 3'UTR inserted downstream of the firefly luciferase showed a significant reduction in reporter activity when cells overexpressed miR146b. As an imbalance in miR146b has been linked to human breast cancers, regulation of STAT3 isoforms may be a novel mechanism by which miR146b participates in breast carcinogenesis.
    URI
    http://hdl.handle.net/1808/10306
    Collections
    • Theses [3711]

    Items in KU ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.


    We want to hear from you! Please share your stories about how Open Access to this item benefits YOU.


    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    Browse

    All of KU ScholarWorksCommunities & CollectionsThis Collection

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    The University of Kansas
      Contact KU ScholarWorks
    Lawrence, KS | Maps
     
    • Academics
    • Admission
    • Alumni
    • Athletics
    • Campuses
    • Giving
    • Jobs

    The University of Kansas prohibits discrimination on the basis of race, color, ethnicity, religion, sex, national origin, age, ancestry, disability, status as a veteran, sexual orientation, marital status, parental status, gender identity, gender expression and genetic information in the University’s programs and activities. The following person has been designated to handle inquiries regarding the non-discrimination policies: Director of the Office of Institutional Opportunity and Access, IOA@ku.edu, 1246 W. Campus Road, Room 153A, Lawrence, KS, 66045, (785)864-6414, 711 TTY.

     Contact KU
    Lawrence, KS | Maps