KUKU

KU ScholarWorks

  • myKU
  • Email
  • Enroll & Pay
  • KU Directory
    • Login
    View Item 
    •   KU ScholarWorks
    • Dissertations and Theses
    • Dissertations
    • View Item
    •   KU ScholarWorks
    • Dissertations and Theses
    • Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    EXERCISE-INDUCED ORGAN PROTECTION IN A MURINE MODEL OF CARDIAC ARREST AND RESUSCITATION

    Thumbnail
    View/Open
    Quinn_ku_0099D_12271_DATA_1.pdf (2.398Mb)
    Issue Date
    2012-08-31
    Author
    Quinn, Carrie
    Publisher
    University of Kansas
    Format
    111 pages
    Type
    Dissertation
    Degree Level
    Ph.D.
    Discipline
    Molecular & Integrative Physiology
    Rights
    This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
    Metadata
    Show full item record
    Abstract
    Cardiac arrest causes whole-body ischemic injury and cell death. Successful cardiopulmonary resuscitation paradoxically confounds recovery by increasing the rate of cellular death and tissue damage through global reperfusion injury. Despite decades of basic and clinical research, the prognosis after a resuscitated cardiac arrest continues to be poor. The substantial effects of cardiac arrest on neurologic function are a major contributor to the high incidence of mortality following resuscitation. Engaging in moderate-intensity aerobic exercise 24 to 72 hours prior to prolonged ischemic exposure can create a prophylactic, conditioned response that minimizes tissue damage. This exercise-induced effect resembles protection conferred by surgical induction of a series of brief, sub-lethal ischemic episodes, known as ischemic preconditioning. The unpredictability of cardiac arrest renders ischemic preconditioning impractical and useless as a neuroprotective defense mechanism. In light of this impracticality, aerobic exercise could provide the only reasonable preventative measure for inducing sustainable organ protection from ischemic injury. Furthermore, characterization of the murine electrocardiogram during and immediately following resuscitated cardiac arrest is exceedingly limited in the current literature. Thus, a clear knowledge gap exists in the methodology of murine models of arrest which we attempted to fill. We hypothesized that exercise preconditioning can confer neuroprotection against prolonged, global ischemia associated with cardiac arrest. Additionally, we proposed that real-time electrocardiographic pattern recognition in the first 30 seconds post-arrest can be used to predict survival in a murine model of cardiac arrest and resuscitation. We tested these hypotheses using male C57Bl/6J mice 10-12 weeks of age in a potassium-induced model of arrest. The mice were trained in a forced treadmill exercise training protocol pre-arrest and neurologic function was serially tested. In the post-arrest period, neurologic testing was repeated to detect changes in cognitive function and emotionality. Our results showed that real-time ECG pattern recognition is a reliable tool for determining the success of resuscitation efforts. Key characteristics of survival emerged in the visual appearance of the RR interval, PR interval, QRS complex, heart rate, and the relation of the J-point to the isoelectric line. These characteristics were substantiated on post-hoc quantitative analysis. Furthermore, as predicted, exercise preconditioning confers neuroprotection during cardiac arrest. This was evidenced by a lower fraction of hippocampal neuronal apoptosis compared with non-exercised animals and a concomitant preservation of retrograde memory.
    URI
    http://hdl.handle.net/1808/10247
    Collections
    • Dissertations [4475]
    • Molecular Biosciences Dissertations and Theses [270]

    Items in KU ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.


    We want to hear from you! Please share your stories about how Open Access to this item benefits YOU.


    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    Browse

    All of KU ScholarWorksCommunities & CollectionsThis Collection

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    The University of Kansas
      Contact KU ScholarWorks
    Lawrence, KS | Maps
     
    • Academics
    • Admission
    • Alumni
    • Athletics
    • Campuses
    • Giving
    • Jobs

    The University of Kansas prohibits discrimination on the basis of race, color, ethnicity, religion, sex, national origin, age, ancestry, disability, status as a veteran, sexual orientation, marital status, parental status, gender identity, gender expression and genetic information in the University’s programs and activities. The following person has been designated to handle inquiries regarding the non-discrimination policies: Director of the Office of Institutional Opportunity and Access, IOA@ku.edu, 1246 W. Campus Road, Room 153A, Lawrence, KS, 66045, (785)864-6414, 711 TTY.

     Contact KU
    Lawrence, KS | Maps