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LC-MS/MS Method for the determination of carbamathione in human plasma
dc.contributor.author | Heemskerk, Anthonius A. M. | |
dc.contributor.author | Haandel, Leon van | |
dc.contributor.author | Wood, Joshua M. | |
dc.contributor.author | McCance-Katz, Elinore F. | |
dc.contributor.author | Williams, Todd D. | |
dc.contributor.author | Stobaugh, John F. | |
dc.contributor.author | Faiman, Morris D. | |
dc.date.accessioned | 2017-05-25T18:44:02Z | |
dc.date.available | 2017-05-25T18:44:02Z | |
dc.date.issued | 2011-03-25 | |
dc.identifier.citation | Heemskerk, A. A. M., van Haandel, L., Woods, J. M., McCance-Katz, E. F., Williams, T. D., Stobaugh, J. F., & Faiman, M. D. (2011). LC-MS/MS Method for the determination of carbamathione in human plasma. Journal of Pharmaceutical and Biomedical Analysis, 54(4), 799–806. http://doi.org/10.1016/j.jpba.2010.10.021 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/24310 | |
dc.description.abstract | Liquid chromatography-tandem mass spectrometry methodology is described for the determination of S-(N,N-diethylcarbamoyl)glutathione (carbamathione) in human plasma samples. Sample preparation consisted of a straightforward perchloric acid medicated protein precipitation, with the resulting supernatant containing the carbamathione (recovery ∼98%). For optimized chromatography/mass spec detection a carbamathione analog, S-(N,N-di-i-propylcarbamoyl)glutathione, was synthesized and used as the internal standard. Carbamathione was found to be stable over the pH 1-8 region over the timeframe necessary for the various operations of the analytical method. Separation was accomplished via reversed-phase gradient elution chromatography with analyte elution and re-equilibration accomplished within 8 minutes. Calibration was established and validated over the concentration range of 0.5-50 nM, which is adequate to support clinical investigations. Intra- and inter-day accuracy and precision determined and found to be < 4% and < 10%, respectively. The methodology was utilized to demonstrate the carbamathione plasma-time profile of a human volunteer dosed with disulfiram (250 mg/d). Interestingly, an unknown but apparently related metabolite was observed with each human plasma sample analyzed. | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 4.0 (CC BY-NC-ND 4.0), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en_US |
dc.subject | LC-MS/MS | en_US |
dc.subject | Carbamathione | en_US |
dc.subject | Disulfuram | en_US |
dc.subject | Glutathione conjugates | en_US |
dc.title | LC-MS/MS Method for the determination of carbamathione in human plasma | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Heemskerk, Anthonius A.M. | |
kusw.kuauthor | van Haandel, Leon | |
kusw.kuauthor | Woods, Joshua M. | |
kusw.kuauthor | Williams, Todd D. | |
kusw.kuauthor | Stobaugh, John F. | |
kusw.kuauthor | Faiman, Morris D. | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
dc.identifier.doi | 10.1016/j.jpba.2010.10.021 | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC4215801 | en_US |
dc.rights.accessrights | openAccess |
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Except where otherwise noted, this item's license is described as: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 4.0 (CC BY-NC-ND 4.0), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.