Show simple item record

dc.contributor.authorDickenson, Nicholas E.
dc.contributor.authorArizmendi Perez, Olivia
dc.contributor.authorPatil, Mrinalini K.
dc.contributor.authorToth, Ronald T., IV
dc.contributor.authorMiddaugh, C. Russell
dc.contributor.authorPicking, William D.
dc.contributor.authorPicking, Wendy Lynn
dc.date.accessioned2017-04-20T16:14:11Z
dc.date.available2017-04-20T16:14:11Z
dc.date.issued2013-12-10
dc.identifier.citationDickenson, N. E., Arizmendi, O., Patil, M. K., Toth, R. T., Middaugh, C. R., Picking, W. D., & Picking, W. L. (2013). The N-terminus of IpaB provides a potential anchor to the Shigella type III secretion system tip complex protein IpaD. Biochemistry, 52(49), 8790–8799. http://doi.org/10.1021/bi400755fen_US
dc.identifier.urihttp://hdl.handle.net/1808/23753
dc.description.abstractThe type III secretion system (T3SS) is an essential virulence factor for Shigella flexneri, providing a conduit through which host-altering effectors are injected directly into a host cell to promote uptake. The type III secretion apparatus (T3SA) is comprised of a basal body, external needle, and regulatory tip complex. The nascent needle is a polymer of MxiH capped by a pentamer of invasion plasmid antigen D (IpaD). Exposure to bile salts (e.g. deoxycholate) causes a conformational change in IpaD and promotes recruitment of IpaB to the needle tip. It has been proposed that IpaB senses contact with host cell membranes, recruiting IpaC and inducing full secretion of T3SS effectors. While the steps of T3SA maturation and their external triggers have been identified, details of specific protein interactions and mechanisms have remained difficult to study due to the hydrophobic nature of the IpaB and IpaC translocator proteins. Here we explored the ability for a series of soluble N-terminal IpaB peptides to interact with IpaD. We found that DOC is required for the interaction and that a region of IpaB between residues 11–27 is required for maximum binding, which was confirmed in vivo. Furthermore, intramolecular FRET measurements indicated that movement of the IpaD distal domain away from the protein core accompanied the binding of IpaB11-226. Together these new findings provide important new insight into the interactions and potential mechanisms that define the maturation of the Shigella T3SA needle tip complex and provide a foundation for further studies probing T3SS activation.en_US
dc.publisherACSen_US
dc.rightsCopyright © 2013 American Chemical Societyen_US
dc.subjectShigellaen_US
dc.subjectType III secretionen_US
dc.subjectDeoxycholateen_US
dc.subjectInvasion plasmid antigenen_US
dc.titleThe N-terminus of IpaB provides a potential anchor to the Shigella type III secretion system tip complex protein IpaDen_US
dc.typeArticleen_US
kusw.kuauthorToth IV, Ronald T.
kusw.kuauthorMiddaugh, C. Russell
kusw.kudepartmentPharmaceutical Chemistryen_US
kusw.oanotesPer SherpaRomeo on 04/20/2017: Author's Pre-print: grey tick subject to Restrictions below, author can archive pre-print (ie pre-refereeing) Restrictions: Must obtain written permission from Editor Must not violate ACS ethical Guidelines Author's Post-print: grey tick subject to Restrictions below, author can archive post-print (ie final draft post-refereeing) Restrictions: If mandated by funding agency or employer/ institution If mandated to deposit before 12 months, must obtain waiver from Institution/Funding agency or use AuthorChoice 12 months embargo Publisher's Version/PDF: cross author cannot archive publisher's version/PDF General Conditions: On author's personal website, pre-print servers, institutional website, institutional repositories or subject repositories Non-Commercial Must be accompanied by set statement (see policy) Must link to publisher version Publisher's version/PDF cannot be useden_US
dc.identifier.doi10.1021/bi400755fen_US
dc.identifier.orcidhttps://orcid.org/0000-0003-1572-6077 https://orcid.org/0000-0001-7998-0643
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccess


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record