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dc.contributor.authorAsad, Nadeem
dc.contributor.authorYih Aw, Wen
dc.contributor.authorTimmons, Lisa
dc.date.accessioned2014-03-07T22:15:33Z
dc.date.available2014-03-07T22:15:33Z
dc.date.issued2012-11-28
dc.identifier.citationAsad, N., Aw, W. Y., & Timmons, L. (2012). Natural and Unanticipated Modifiers of RNAi Activity in Caenorhabditis elegans. PLoS ONE, 7(11). http://dx.doi.org/10.1371/journal.pone.0050191
dc.identifier.urihttp://hdl.handle.net/1808/13164
dc.description.abstractOrganisms used as model genomics systems are maintained as isogenic strains, yet evidence of sequence differences between independently maintained wild-type stocks has been substantiated by whole-genome resequencing data and strain-specific phenotypes. Sequence differences may arise from replication errors, transposon mobilization, meiotic gene conversion, or environmental or chemical assault on the genome. Low frequency alleles or mutations with modest effects on phenotypes can contribute to natural variation, and it has proven possible for such sequences to become fixed by adapted evolutionary enrichment and identified by resequencing. Our objective was to identify and analyze single locus genetic defects leading to RNAi resistance in isogenic strains of Caenorhabditis elegans. In so doing, we uncovered a mutation that arose de novo in an existing strain, which initially frustrated our phenotypic analysis. We also report experimental, environmental, and genetic conditions that can complicate phenotypic analysis of RNAi pathway defects. These observations highlight the potential for unanticipated mutations, coupled with genetic and environmental phenomena, to enhance or suppress the effects of known mutations and cause variation between wild-type strains.
dc.publisherPublic Library of Science
dc.subjectAlleles
dc.subjectCaenorhabditis elegans
dc.subjectDNA sequences
dc.subjectInsertion Mutation
dc.subjectRNA sequencing
dc.subjectSilent Mutation
dc.subjectSmall Interfering RNA
dc.subjectTransposable elements
dc.titleNatural and Unanticipated Modifiers of RNAi Activity in Caenorhabditis elegans
dc.typeArticle
kusw.kuauthorAsad, Nadeem
kusw.kuauthorYih Aw, Wen
kusw.kuauthorTimmons, Lisa
kusw.kudepartmentNatural and Unanticipated Modifiers of RNAi Activity in Caenorhabditis elegans
kusw.oastatusfullparticipation
dc.identifier.doi10.1371/journal.pone.0050191
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item meets KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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