Immunogenicity and protective efficacy of nanoparticle formulations of L-SseB against Salmonella infection
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Issue Date
2023-06-30Author
Das, Sayan
Howlader, Debaki R.
Lu, Ti
Whittier, Sean K.
Hu, Gang
Sharma, Simran
Dietz, Zackary K.
Ratnakaram, Siva S. K.
Varisco, David J.
Ernst, Robert K.
Picking, William D.
Picking, Wendy L.
Publisher
Frontiers Media
Type
Article
Article Version
Scholarly/refereed, publisher version
Rights
© 2023 Das, Howlader, Lu, Whittier, Hu, Sharma, Dietz, Ratnakaram, Varisco, Ernst, Picking and Picking. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
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Show full item recordAbstract
Salmonella enterica, a Gram-negative pathogen, has over 2500 serovars that infect a wide range of hosts. In humans, S. enterica causes typhoid or gastroenteritis and is a major public health concern. In this study, SseB (the tip protein of the Salmonella pathogenicity island 2 type III secretion system) was fused with the LTA1 subunit of labile-toxin from enterotoxigenic E. coli to make the self-adjuvanting antigen L-SseB. Two unique nanoparticle formulations were developed to allow multimeric presentation of L-SseB. Mice were vaccinated with these formulations and protective efficacy determined via challenging the mice with S. enterica serovars. The polysaccharide (chitosan) formulation was found to elicit better protection when compared to the squalene nanoemulsion. When the polysaccharide formulation was used to vaccinate rabbits, protection from S. enterica challenge was elicited. In summary, L-SseB in a particulate polysaccharide formulation appears to be an attractive candidate vaccine capable of broad protection against S. enterica.
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Citation
Das S, Howlader DR, Lu T, Whittier SK, Hu G, Sharma S, Dietz ZK, Ratnakaram SSK, Varisco DJ, Ernst RK, Picking WD and Picking WL (2023) Immunogenicity and protective efficacy of nanoparticle formulations of L-SseB against Salmonella infection. Front. Immunol. 14:1208848. doi: 10.3389/fimmu.2023.1208848
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