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Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids

dc.contributor.authorAsad, Nadeem
dc.contributor.authorSmith, Emily
dc.contributor.authorShakya, Sudeep
dc.contributor.authorStegman, Sutton
dc.contributor.authorTimmons, Lisa
dc.date.accessioned2023-04-11T18:02:18Z
dc.date.available2023-04-11T18:02:18Z
dc.date.issued2023-03-06
dc.identifier.citationAsad, N.; Smith, E.; Shakya, S.; Stegman, S.; Timmons, L. Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids. Methods Protoc. 2023, 6, 25. https://doi.org/10.3390/mps6020025en_US
dc.identifier.urihttps://hdl.handle.net/1808/34089
dc.description.abstractWe describe a workflow for efficient, environmentally attentive, and sustainable practices related to routine agarose gel electrophoresis. The methods reduce plastic waste and improve efficiency, especially for the exhaustive screening of difficult-to-obtain plasmids. Sustainability is increased when agarose is used ten times over by virtue of a thorough recycling regimen. The workflow optimizes workspaces and standardizes lab practices for handling potentially hazardous waste, minimizing environmental harm. Safety, efficiency, and sustainability improve laboratory productivity, help minimize environmental contamination, and increase cost-effectiveness.en_US
dc.publisherMDPIen_US
dc.rights© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectElectrophoresisen_US
dc.subjectAgaroseen_US
dc.subjectEthidium bromideen_US
dc.subjectSustainabilityen_US
dc.titleSustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmidsen_US
dc.typeArticleen_US
kusw.kuauthorAsad, Nadeem
kusw.kuauthorSmith, Emily
kusw.kuauthorShakya, Sudeep
kusw.kuauthorStegman, Sutton
kusw.kuauthorTimmons, Lisa
kusw.kudepartmentMolecular Biosciencesen_US
dc.identifier.doi10.3390/mps6020025en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-4460-0636en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-8651-9174en_US
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccessen_US


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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
Except where otherwise noted, this item's license is described as: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.