A Multilaboratory Comparison of Calibration Accuracy and the Performance of External References in Analytical Ultracentrifugation
dc.contributor.author | Zhao, Huaying | |
dc.contributor.author | Ghirlando, Rodolfo | |
dc.contributor.author | Alfonso, Carlos | |
dc.contributor.author | Arisaka, Fumio | |
dc.contributor.author | Attali, Ilan | |
dc.contributor.author | Bain, David L. | |
dc.contributor.author | Bakhtina, Marina M. | |
dc.contributor.author | Becker, Donald F. | |
dc.contributor.author | Bedwell, Gregory J. | |
dc.contributor.author | Bekdemir, Ahmet | |
dc.contributor.author | Besong, Tabot M. D. | |
dc.contributor.author | Birck, Catherine | |
dc.contributor.author | Brautigam, Chad A. | |
dc.contributor.author | Brennerman, William | |
dc.contributor.author | Byron, Owlyn | |
dc.contributor.author | Bzowska, Agnieszka | |
dc.contributor.author | Chaires, Johnathan B. | |
dc.contributor.author | Chaton, Catherine T. | |
dc.contributor.author | Coelfen, Helmut | |
dc.contributor.author | Connaghan, Keith D. | |
dc.contributor.author | Crowley, Kimberly A. | |
dc.contributor.author | Curth, Ute | |
dc.contributor.author | Daviter, Tina | |
dc.contributor.author | Dean, William L. | |
dc.contributor.author | Diez, Ana I. | |
dc.contributor.author | Ebel, Christine | |
dc.contributor.author | Eckert, Debra M. | |
dc.contributor.author | Eisele, Leslie E. | |
dc.contributor.author | Eisenstein, Edward | |
dc.contributor.author | England, Patrick | |
dc.contributor.author | Escalante, Carlos | |
dc.contributor.author | Fagan, Jeffery A. | |
dc.contributor.author | Fairman, Robert | |
dc.contributor.author | Finn, Ron M. | |
dc.contributor.author | Fischle, Wolfgang | |
dc.contributor.author | de la Torre, Jose Garcia | |
dc.contributor.author | Gor, Jayesh | |
dc.contributor.author | Gustafsson, Henning | |
dc.contributor.author | Hall, Damien | |
dc.contributor.author | Harding, Stephen E. | |
dc.contributor.author | Cifre, Jose G. Hernadez | |
dc.contributor.author | Herr, Andrew B. | |
dc.contributor.author | Howell, Elizebeth E. | |
dc.contributor.author | Isaac, Richard S. | |
dc.contributor.author | Jao, Shu-Chuan | |
dc.contributor.author | Jose, Davis | |
dc.contributor.author | Kim, Soon-Jong | |
dc.contributor.author | Kokona, Bashkim | |
dc.contributor.author | Kornblatt, Jack A. | |
dc.contributor.author | Kosek, Dalibor | |
dc.contributor.author | Krayukhina, Elena | |
dc.contributor.author | Krzizike, Daniel | |
dc.contributor.author | Kusznir, Eric A. | |
dc.contributor.author | Kwon, Hyewon | |
dc.contributor.author | Larson, Adam | |
dc.contributor.author | Laue, Thomas M. | |
dc.contributor.author | Le Roy, Aline | |
dc.contributor.author | Leech, Andrew P. | |
dc.contributor.author | Lilie, Hauke | |
dc.contributor.author | Luger, Karolin | |
dc.contributor.author | Luque-Ortega, Juan R. | |
dc.contributor.author | Ma, Jia | |
dc.contributor.author | May, Carrie A. | |
dc.contributor.author | Maynard, Ernest L. | |
dc.contributor.author | Modrak-Wojcik, Anna | |
dc.contributor.author | Mok, Yee-Foong | |
dc.contributor.author | Muecke, Norbert | |
dc.contributor.author | Nagel-Steger, Luitgard | |
dc.contributor.author | Narlikar, Geeta J. | |
dc.contributor.author | Noda, Masanori | |
dc.contributor.author | Nourse, Amanda | |
dc.contributor.author | Obsil, Tomas | |
dc.contributor.author | Park, Chad K. | |
dc.contributor.author | Park, Jin-Ku | |
dc.contributor.author | Pawelek, Peter D. | |
dc.contributor.author | Perdue, Erby E. | |
dc.contributor.author | Perkins, Stephen J. | |
dc.contributor.author | Perugini, Matthew A. | |
dc.contributor.author | Peterson, Craig L. | |
dc.contributor.author | Peverelli, Martin G. | |
dc.contributor.author | Piszczek, Grzegorz | |
dc.contributor.author | Prag, Gali | |
dc.contributor.author | Prevelige, Peter E. | |
dc.contributor.author | Raynal, Bertrand D. E. | |
dc.contributor.author | Rezabkova, Lenka | |
dc.contributor.author | Richter, Klaus | |
dc.contributor.author | Ringel, Alison E. | |
dc.contributor.author | Rosenberg, Rose | |
dc.contributor.author | Rowe, Arthur J. | |
dc.contributor.author | Rufer, Arne C. | |
dc.contributor.author | Scott, David J. | |
dc.contributor.author | Seravalli, Javier G. | |
dc.contributor.author | Solovyova, Alexandra S. | |
dc.contributor.author | Song, Renjie | |
dc.contributor.author | Staunton, David | |
dc.contributor.author | Stoddard, Caitlin | |
dc.contributor.author | Stott, Katherine | |
dc.contributor.author | Strauss, Holger M. | |
dc.contributor.author | Streicher, Werner W. | |
dc.contributor.author | Sumida, John P. | |
dc.contributor.author | Swygert, Sarah G. | |
dc.contributor.author | Szczepanowski, Roman H. | |
dc.contributor.author | Tessmer, Ingrid | |
dc.contributor.author | Toth, Ronald T., IV | |
dc.contributor.author | Tripathy, Ashutosh | |
dc.contributor.author | Uchiyama, Susumu | |
dc.contributor.author | Uebel, Stephan F. W. | |
dc.contributor.author | Unazi, Satoru | |
dc.contributor.author | Gruben, Anna Vitlin | |
dc.contributor.author | von Hippel, Peter H. | |
dc.contributor.author | Wandrey, Christine | |
dc.contributor.author | Wang, Szu-Huan | |
dc.contributor.author | Weitzel, Steven E. | |
dc.contributor.author | Wielgu-Kutrowska, Beata | |
dc.contributor.author | Wolberger, Cynthia | |
dc.contributor.author | Wolff, Martin | |
dc.contributor.author | Wright, Edward | |
dc.contributor.author | Yu-Sung, Wu | |
dc.contributor.author | Wubben, Jacinta M. | |
dc.contributor.author | Schuck, Peter | |
dc.date.accessioned | 2015-06-15T18:14:30Z | |
dc.date.available | 2015-06-15T18:14:30Z | |
dc.date.issued | 2015-05-21 | |
dc.identifier.citation | Zhao H, Ghirlando R, Alfonso C, Arisaka F, Attali I, Bain DL, et al. (2015) A Multilaboratory Comparison of Calibration Accuracy and the Performance of External References in Analytical Ultracentrifugation. PLoS ONE 10(5): e0126420. http://dx.doi.org/10.1371/journal.pone.0126420 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/18067 | |
dc.description.abstract | Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules and their complexes, reporting on their size and shape in free solution. The purpose of this multi-laboratory study was to establish the precision and accuracy of basic data dimensions in AUC and validate previously proposed calibration techniques. Three kits of AUC cell assemblies containing radial and temperature calibration tools and a bovine serum albumin (BSA) reference sample were shared among 67 laboratories, generating 129 comprehensive data sets. These allowed for an assessment of many parameters of instrument performance, including accuracy of the reported scan time after the start of centrifugation, the accuracy of the temperature calibration, and the accuracy of the radial magnification. The range of sedimentation coefficients obtained for BSA monomer in different instruments and using different optical systems was from 3.655 S to 4.949 S, with a mean and standard deviation of (4.304 ± 0.188) S (4.4%). After the combined application of correction factors derived from the external calibration references for elapsed time, scan velocity, temperature, and radial magnification, the range of s-values was reduced 7-fold with a mean of 4.325 S and a 6-fold reduced standard deviation of ± 0.030 S (0.7%). In addition, the large data set provided an opportunity to determine the instrument-to-instrument variation of the absolute radial positions reported in the scan files, the precision of photometric or refractometric signal magnitudes, and the precision of the calculated apparent molar mass of BSA monomer and the fraction of BSA dimers. These results highlight the necessity and effectiveness of independent calibration of basic AUC data dimensions for reliable quantitative studies. | en_US |
dc.description.sponsorship | This work was supported by the Intramural Research Programs of the National Institute of Biomedical Imaging and Bioengineering, the National Heart, Lung, and Blood Institute, and the National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health; the Max Planck Society; and the Rudolf Virchow Center for Experimental Biomedicine by the German Research Council (Deutsche Forschungsgemeinschaft, DFG) FZ 82. Further, this work was supported by National Cancer Institute grant CA35635, grants from the National Institute of General Medical Sciences (GM 095822, GM 109102, GM 094363, and P30GM103519), and by grant CTQ2012-33717 from MINECO-Spain/FEDER. The purchase of the XL-I AUC instrument for IIMCB Warsaw was supported by Centre for Preclinical Research and Technology (CePT) - European Union POIG.02.02.00-14-024/08-00 project. This work used the platforms of the Grenoble Instruct centre (ISBG; UMS 3518 CNRS-CEA-UJF-EMBL) with support from FRISBI (ANR-10-INSB-05-02) and GRAL (ANR-10-LABX-49-01) within the Grenoble Partnership for Structural Biology (PSB). This work was supported in part by NIH research grants GM-15792 and GM-29158 and by a Shared Instrument Proposal grant from NSF (P. H. von Hippel, Principle Investigator). The work in the Strasbourg centre was supported by the French Infrastructure for Integrated Structural Biology (FRISBI) ANR-10-INSB-05-01, and Instruct as part of the European Strategy Forum on Research Infrastructures (ESFRI). Some of the experiments in the present study were performed in the NanoFun laboratories co-financed by the European Regional Development Fund within the Innovation Economy Operational Program, Project No. POIG.02.02.00-00-025/09. S-JK acknowledges support from National Research Foundation of Korea (2011-0010437). LR acknowledges the receipt of EMBO long-term and Marie Curie IEF fellowships. DH acknowledges the receipt of an A.N.U. Senior Research Fellowship. DME and the University of Utah Protein Interaction Core Facility are supported by NIH Grant GM82545. KL acknowledges NIH grant GM067777, and DK acknowledges NIH grant F31GM105363. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | en_US |
dc.publisher | Public Library of Science | en_US |
dc.rights | This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication | |
dc.rights.uri | https://creativecommons.org/publicdomain/zero/1.0/ | |
dc.subject | Rotors | en_US |
dc.subject | Sdimentation | en_US |
dc.subject | Data acquisation | en_US |
dc.subject | Istrument calibration | en_US |
dc.subject | Government laboratory | en_US |
dc.subject | Convection | en_US |
dc.subject | Viscosity | en_US |
dc.subject | Statistical data | en_US |
dc.title | A Multilaboratory Comparison of Calibration Accuracy and the Performance of External References in Analytical Ultracentrifugation | en_US |
dc.type | Article | |
kusw.kuauthor | Toth, Ronald T. IV | |
kusw.kudepartment | Department of Pharmaceutical Chemistry | en_US |
dc.identifier.doi | 10.1371/journal.pone.0126420 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item meets KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess |
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