dc.contributor.author | Priyadarshi, Amit | |
dc.contributor.author | Tang, Liang | |
dc.date.accessioned | 2015-05-05T20:21:57Z | |
dc.date.available | 2015-05-05T20:21:57Z | |
dc.date.issued | 2010-11-01 | |
dc.identifier.citation | Priyadarshi, A., & Tang, L. (2010). Crystallization and preliminary X-ray crystallographic analysis of the Type III secretion translocator chaperone SicA from Salmonella enterica. Acta Crystallogr Sect F Struct Biol Cryst Commun, 66(11), 1533-1535. http://www.dx.doi.org/10.1107/S1744309110037954 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/17599 | |
dc.description | This is the publisher's version, also available electronically from "http://scripts.iucr.org". | en_US |
dc.description.abstract | SicA is a member of the class II chaperones in type III secretion systems which bind to the pore-forming translocators in the bacterial cytoplasm and prevent them from premature association and degradation. In this study, SicA from Salmonella enterica serovar Typhimurium was overexpressed, purified and crystallized using PEG 8000 as the precipitant. X-ray diffraction data were collected using synchrotron radiation and processed at 3.5 Å resolution. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a = 180.4, b = 94.1, c = 131.8 Å, [beta] = 130.9°. There may be eight monomers in the crystallographic asymmetric unit, corresponding to a VM of 2.52 Å3 Da-1 and a solvent content of 51.1%. This suggests an oligomerization state that differs from those of previously reported type III secretion chaperones. | en_US |
dc.publisher | International Union of Crystallography | en_US |
dc.title | Crystallization and preliminary X-ray crystallographic analysis of the Type III secretion translocator chaperone SicA from Salmonella enterica | en_US |
dc.type | Article | |
kusw.kuauthor | Tang, Liang | |
kusw.kudepartment | Molecular Biosciences | en_US |
dc.identifier.doi | 10.1107/S1744309110037954 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item meets KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess | |