dc.contributor.author | Woolley, G. Andrew | |
dc.contributor.author | Zunic, Valentin | |
dc.contributor.author | Karanicolas, John | |
dc.contributor.author | Jaikaran, Anna S. I. | |
dc.contributor.author | Starostin, Andrei V. | |
dc.date.accessioned | 2015-04-24T17:13:47Z | |
dc.date.available | 2015-04-24T17:13:47Z | |
dc.date.issued | 1997-11 | |
dc.identifier.citation | Woolley, G.a., V. Zunic, J. Karanicolas, A.s. Jaikaran, and A.v. Starostin. "Voltage-dependent Behavior of a "ball-and-chain" Gramicidin Channel." Biophysical Journal 73.5 (1997): 2465-475. http://dx.doi.org/10.1016/S0006-3495(97)78275-4. | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/17523 | |
dc.description | This is the published version. Copyright 1997 by Elsevier. | en_US |
dc.description.abstract | The channel-forming properties of two analogs of gramicidin, gramicidin-ethylenediamine (gram-EDA), and gramicidin-N,N-dimethylethylenediamine (gram-DMEDA) were studied in planar lipid bilayers, using protons as the permeant ion. These peptides have positively charged amino groups tethered to their C-terminal ends via a linker containing a carbamate group. Gram-DMEDA has two extra methyl groups attached to the terminal amino group, making it a bulkier derivative. The carbamate groups undergo thermal cis-trans isomerization on the 10–100-ms time scale. The conductance behavior of gram-EDA is found to be markedly voltage dependent, whereas the behavior of gram-DMEDA is not. In addition, voltage affects the cis-trans ratios of the carbamate groups of gram-EDA, but not those of gram-DMEDA. A model is proposed to account for these observations, in which voltage can promote the binding of the terminal amino group of gram-EDA to the pore in a "ball-and-chain" fashion. The bulkiness of the gram-DMEDA derivative prevents this binding. | en_US |
dc.publisher | The Biophysical Society | en_US |
dc.title | Voltage-dependent behavior of a "ball-and-chain" gramicidin channel | en_US |
dc.type | Article | |
kusw.kuauthor | Karanicolas, John | |
kusw.kudepartment | Molecular Biosciences | en_US |
dc.identifier.doi | 10.1016/S0006-3495(97)78275-4 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item does not meet KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess | |