Integrated immunoisolation and protein analysis of circulating exosomes using microfluidic technology
dc.contributor.author | He, Mei | |
dc.contributor.author | Crow, Jennifer M. | |
dc.contributor.author | Roth, Marc | |
dc.contributor.author | Zeng, Yong | |
dc.contributor.author | Godwin, Andrew K. | |
dc.date.accessioned | 2014-11-14T19:50:21Z | |
dc.date.available | 2014-11-14T19:50:21Z | |
dc.date.issued | 2014-08-06 | |
dc.identifier.citation | Mei He, Jennifer Crow, Marc Roth, Yong Zeng, Andrew K. Godwin. "Integrated immunoisolation and protein analysis of circulating exosomes using microfluidic technology." Lab Chip. 2014 October 7; 14(19): 3773–3780. Published online 2014 August 6. http://dx.doi.org/10.1039/c4lc00662c | |
dc.identifier.uri | http://hdl.handle.net/1808/15759 | |
dc.description.abstract | Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy is difficult, costly, and sometimes not even an option. Tumor-derived exosomes have attracted increasing interest in non-invasive cancer diagnosis and monitoring of treatment response. However, the biology and clinical value of exosomes remains largely unknown due in part to current technical challenges in rapid isolation, molecular classification and comprehensive analysis of exosomes. Here we developed a new microfluidic approach to streamline and expedite the exosome analysis pipeline by integrating specific immunoisolation and targeted protein analysis of circulating exosomes. Compared to the conventional methods, our approach enables selective subpopulation isolation and quantitative detection of surface and intravesicular biomarkers directly from a minimally invasive amount of plasma samples (30 μL) within ~100 min with markedly improved detection sensitivity. Using this device, we demonstrated phenotyping of exosome subpopulations by targeting a panel of common exosomal and tumor-specific markers and multiparameter analyses of intravesicular biomarkers in the selected subpopulation. We were able to assess the total expression and phosphorylation levels of IGF-1R in non-small-cell lung cancer patients by probing plasma exosomes as a non-invasive alternative to conventional tissue biopsy. We foresee that the microfluidic exosome analysis platform will form the basis for critically needed infrastructures for advancing the biology and clinical utilization of exosomes. | |
dc.publisher | RSC Publsihing | |
dc.rights | This journal is © The Royal Society of Chemistry 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/2.0/uk/ | |
dc.title | Integrated immunoisolation and protein analysis of circulating exosomes using microfluidic technology | |
dc.type | Article | |
kusw.kuauthor | Zeng, Yong | |
kusw.kudepartment | Deparment of Chemistry | |
kusw.oastatus | fullparticipation | |
dc.identifier.doi | 10.1039/c4lc00662c | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item meets KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess |
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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.