Gunasekara, Dulan B.Hulvey, Matthew K.Lunte, Susan M.da Silva, José A. F.2017-03-312017-03-312012-03-14Gunasekara, D. B., Hulvey, M. K., Lunte, S. M., & da Silva, J. A. F. (2012). Microchip electrophoresis with amperometric detection for the study of the generation of nitric oxide by NONOate salts. Analytical and Bioanalytical Chemistry, 403(8), 2377–2384. http://doi.org/10.1007/s00216-012-5810-4https://hdl.handle.net/1808/23560Microchip electrophoresis (ME) with electrochemical detection was used to monitor nitric oxide (NO) production from diethylammonium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate (DEA/NO) and 1-(hydroxyl-NNO-azoxy)-L-proline disodium salt (PROLI/NO). NO was generated through acid hydrolysis of these NONOate salts. The products of acid hydrolysis were introduced into a 5-cm separation channel using gated injection. The separation was accomplished using reverse polarity and a background electrolyte consisting of 10 mM boric acid and 2 mM tetradecyltrimethylammonium bromide, pH 11. Electrochemical detection was performed using an isolated potentiostat in an in-channel configuration. Potentials applied to the working electrode, typically higher than +1.0 V vs. Ag/AgCl, allowed the direct detection of nitrite, NO, DEA/NO, and PROLI/NO. Baseline resolution was achieved for the separation of PROLI/NO and NO while resolution between DEA/NO and NO was poor (1.0±0.2). Nitrite was present in all samples tested.© Springer-Verlag 2012Nitric oxideMicrochip electrophoresisReactive nitrogen speciesAmperometric detectionNONOatesArticle10.1007/s00216-012-5810-4https://orcid.org/0000-0001-9522-6181openAccess