Middaugh, C. RussellKissmann, Julian Michael2010-06-092010-06-092010-04-202010http://dissertations.umi.com/ku:10875https://hdl.handle.net/1808/6299This dissertation describes the application of a composite biophysical approach to the characterization and stabilization of viruses and virus-like particles for vaccine purposes. Spectroscopic, calorimetric, and light-scattering techniques were applied to detect physical changes in multiple aspects of viral architecture following the application of pharmaceutically relevant stress factors. The signals from a variety of these techniques were then combined in a vector-based approach to generate empirical phase diagrams that visually depict changes in each system's physical state as a function of experimental stress factors. These diagrams were then used to determine optimal solution storage conditions and also conditions for screening excipient libraries for appropriate stabilizers.156 pagesENThis item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.Pharmaceutical chemistryFormulationInfluenzaMeaslesStabilizationVaccineVirus-like particlesDissertationopenAccess