Masson, Glenn R.Burke, John E.Ahn, Natalie G.Anand, Ganesh S.Borchers, ChristophBrier, SébastienBou-Assaf, George M.Engen, John R.Englander, S. WalterFaber, JohanGarlish, RachelGriffin, Patrick R.Gross, Michael L.Guttman, MiklosHamuro, YoshitomoHeck, Albert J. R.Houde, DamianIacob, Roxana E.Jørgensen, Thomas J. D.Kaltashov, Igor A.Klinman, Judith P.Konermann, LarsMan, PetrMayne, LelandPascal, Bruce D.Reichmann, DanaSkehel, MarkSnijder, JoostStrutzenberg, Timothy S.Underbakke, Eric S.Wagner, CorneliaWales, Thomas E.Walters, Benjamin T.Weis, David D.Wilson, Derek J.Wintrode, Patrick L.Zhang, ZhongqiZheng, JieSchriemer, David C.Rand, Kasper D.2019-11-072019-11-072019-06-27Masson, G. R., Burke, J. E., Ahn, N. G., Anand, G. S., Borchers, C., Brier, S., … Rand, K. D. (2019). Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments. Nature methods, 16(7), 595–602. doi:10.1038/s41592-019-0459-yhttps://hdl.handle.net/1808/29728Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical technique being increasingly applied to a wide variety of problems. As the HDX-MS community continues to grow, adoption of best practices in data collection, analysis, presentation and interpretation will greatly enhance the accessibility of this technique to nonspecialists. Here we provide recommendations arising from community discussions emerging out of the first International Conference on Hydrogen-Exchange Mass Spectrometry (IC-HDX; 2017). It is meant to represent both a consensus viewpoint and an opportunity to stimulate further additions and refinements as the field advances.© The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.http://creativecommons.org/licenses/by/4.0/.Article10.1038/s41592-019-0459-yhttps://orcid.org/0000-0002-1386-4719https://orcid.org/0000-0001-7904-9859https://orcid.org/0000-0002-6918-9476https://orcid.org/0000-0003-1159-4636https://orcid.org/0000-0002-2405-4404https://orcid.org/0000-0001-6106-4698https://orcid.org/0000-0001-5734-2843https://orcid.org/0000-0003-0315-5334https://orcid.org/0000-0003-0598-534Xhttps://orcid.org/0000-0003-4269-7339https://orcid.org/0000-0003-3032-1211https://orcid.org/0000-0002-7012-6085https://orcid.org/0000-0002-5202-1618https://orcid.org/0000-0002-6337-5489PMC6614034OpenAccess