Trivedi, MaulikDavis, R. AndrewShabaik, YumnaRoy, AmbrishVerkhivker, Gennady M.Laurence, Jennifer S.Middaugh, C. RussellSiahaan, Teruna J.2017-04-262017-04-262009-10Trivedi, M., Davis, R. A., Shabaik, Y., Roy, A., Verkhivker, G., Laurence, J., … Siahaan, T. J. (2009). The role of covalent dimerization on the physical and chemical stability of the EC1 domain of human E-cadherin. Journal of Pharmaceutical Sciences, 98(10), 3562–3574. http://doi.org/10.1002/jps.21686https://hdl.handle.net/1808/23815The objective of this work was to evaluate the solution stability of the EC1 domain of E-cadherin under various conditions. The EC1 domain was incubated at various temperatures (4, 37, and 70 °C) and pH values (3.0, 7.0, and 9.0). At pH 9.0 and 37 or 70 °C, a significant loss of EC1 was observed due to precipitation and a hydrolysis reaction. The degradation was suppressed upon addition of DTT, suggesting that the formation of EC1 dimer facilitated the EC1 degradation. At 4 °C and various pH values, the EC1 secondary and tertiary showed changes upon incubation up to 28 days, and DTT prevented any structural changes upon 28 days of incubation. Molecular dynamics simulations indicated that the dimer of EC1 has higher mobility than does the monomer; this higher mobility of the EC1 dimer may contribute to instability of the EC1 domain.This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 4.0 (CC BY-NC-ND 4.0), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.http://creativecommons.org/licenses/by-nc-nd/4.0/Article10.1002/jps.21686PMC3319692openAccess